1887

Abstract

Purified deoxyribonucleic acids (DNAs) of representative strains of nine established serovars of the human mycoplasma and of five strains of were digested with a variety of restriction endonucleases. The cleavage patterns obtained by electrophoresis of the digestion products separated the nine serovars into two definite clusters, one consisting of serovars 1, 3, and 6 and the other consisting of serovars 2, 4, 5, 7, 8, and 9, in agreement with previous results obtained by electrophoresis of cell proteins and DNA-DNA hybridization. Although the five strains differed in virulence and adherence capacity, they exhibited similar DNA cleavage patterns, indicating a high degree of genetic homogeneity. The enzymes I, I, HI, and I, which possess guanine-plus-cytosine-rich recognition sequences, cleaved the DNA of (guanine-plus-cytosine content, about 28 mol%) into a small number of fragments, whereas I, with the recognition sequence CCC/GGG, failed to cleave DNA into visibly distinct fragments. The same restriction enzymes produced multiband cleavage patterns with DNA, which has a guanine-plus-cytosine content of about 40 mol%. Restriction endonucleases RI, dIII, I, and I, which have recognition sequences rich in adenine plus thy mine, produced multiband patterns with the DNAs of both and We concluded that the cleavage patterns of mycoplasmal DNAs digested with restriction endonucleases provide a means for determining genetic relatedness among mycoplasmas.

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1983-04-01
2022-10-01
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