1887

Abstract

strains whose taxonomic position is uncertain were examined in detail. The lecithinases of these strains exhibited less avidity to the alpha-antitoxin of than did the lecithinase. On the basis of a computer analysis, the strains were grouped into two phenons, I and III, both of which are distinctly separable from C. (phenon II). Strains previously identified by us as belonging to Nakamura et al. 1970, including strain G (= ATCC 27639), here designated as the type strain of , were found to belong to phenon I, and the strains of phenon III are regarded as constituting a new species, for which we propose the name . The type strain of is HA-7103 (= ATCC 27555). The main characters differentiating from are as follows: produces a lecithinase which exhibits extremely low avidity to alpha-antitoxin, rapidly ferments salicin, does not ferment raffinose, and does not liquefy 10% gelatin; the main characters of which differentiate it from phenon I strains are: larger cell width, a distinctly stronger lecithinase reaction, liquefaction of 2% gelatin, production of butanol, and weak toxicity for mice. Deoxyribonucleic acid (DNA)-DNA homology studies of phenons I, II, and III confirmed the validity of the above-mentioned groupings. A computer analysis of other saccharolytic clostridia revealed that , and are associated by similarity values higher than 90% and that and constitute separate, but closely related, taxa.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-23-4-419
1973-10-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/23/4/ijs-23-4-419.html?itemId=/content/journal/ijsem/10.1099/00207713-23-4-419&mimeType=html&fmt=ahah

References

  1. Batty I., Walker P. D. 1963; Differentiation of Clostridium septicum and Clostridium chauvoei by the use of fluorescent antibodies. J. Pathol. Bacteriol 35:517–521
    [Google Scholar]
  2. Beers R. J., Fischer J., Magraw B. S., Lockhart W. R. 1965; A comparison of methods for computer taxonomy. J. Gen. Microbiol 28:641–652
    [Google Scholar]
  3. Breed R. S., Murray E. G. D., Smith N. R. 1957; Bergey’s manual of determinative bacteriology. , 7.677–678 Williams & Wilkins; Baltimore:
    [Google Scholar]
  4. Brooks M. E., Epps H. B. G. 1959; Taxonomic studies of the genus Clostridium: Clostridium bifermentans and Clostridium sordellii. J. Gen. Microbiol 21:144–155
    [Google Scholar]
  5. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: Clostridium butyri-cium and other butyric acid-producing clostridia. J. Gen. Microbiol 67:33–46
    [Google Scholar]
  6. Evans D. G. 1945; The in-vivo production of alpha-toxin, theta-haemolysin and hyaluronidase by strains of Clostridium welchii type A and the relationship of in-vitro properties to virulence in guinea pigs. J. Pathol. Bacteriol 57:75–85
    [Google Scholar]
  7. Grob D. 1964; Proteolytic enzymes. I. The control of their activity. J. Gen. Physiol 29:219–245
    [Google Scholar]
  8. Hanaoka M., Kato Y., Amano T. 1969; Complementary examination of DNA’s among Vibrio species. Biken J 12:181–185
    [Google Scholar]
  9. Keppie J., Robertson M. 1944; The in-vitro toxigenicity and other characters of strains of Cl. welchii type A from wounds sources. J. Bacteriol. Pathol 56:123–132
    [Google Scholar]
  10. McCarthy B. J., Bolton E. T. 1963; An approach to the measurement of genetic related-ness among organisms. Proc. Nat. Acad. SciU.S.A 50:156–164
    [Google Scholar]
  11. McCarthy B. J., Hoyer B. H. 1964; Identity of DNA and diversity of messenger RNA molecules in normal mouse tissues. Proc. Nat. Acad. SciU.S.A 52:915–922
    [Google Scholar]
  12. Marmur J. 1961; A principle for the isolation of desoxyribonucleic acid from microorganisms. J. Mol.Biol 3:208–218
    [Google Scholar]
  13. Moore W. E. C., Cato E. P., Holdeman L. V. 1966; Fermentation patterns of some clostridia species. Int. J. Syst. Bacteriol 16:383–415
    [Google Scholar]
  14. Moussa R. S. 1959; Complexity of toxins from Clostridium septicum and Clostridium chauvoei. J. Bacteriol 76:583–545
    [Google Scholar]
  15. Nakamura S., Tamai K., Nishida S. 1970; Criteria for identification of Clostridium perfringens. 6. Clostridium paraperfringens sp. nov. Med.Biol 80:137–140
    [Google Scholar]
  16. Nishida S., Murakami M., Yamagishi T. 1962; Chopped meat broth as a medium for the production of toxins by clostridia. I. Production of toxins by Clostridium welchii. Jap. J. Microbiol 6:33–40
    [Google Scholar]
  17. Prévot A. R. 1957; Manual de classification et de détermination des bactéries anaérobies. 162–163 Masson et Compagnie; Paris:
    [Google Scholar]
  18. Prévot A. R., Raynaud M. 1944; Sur une nouvelle éspece anaerobié isolée de l’huitre. Inflabilis setiensis sp. n. Ann. Inst. Past 70:50–51
    [Google Scholar]
  19. Prévot A. R., Thouvenot H., Patricalla M., Silloc R. 1956; Une nouvelle éspece anaerobié des lacs du Ruwenzor: Inflabilis lacustris sp.n. Ann. Inst. Past 91:929–932
    [Google Scholar]
  20. Prévot A. R., Turpin A., Kaiser P. 1967; Les bactéries anaérobies. 606 Dunod; Paris:
    [Google Scholar]
  21. Roberts T. A. 1967; Sporulation of mesophilic clostridia. J. Appl. Bacteriol 30:430–443
    [Google Scholar]
  22. Sneath P. H. A. 1957; The application of computers to taxonomy. J. Gen. Microbiol 17:201–221
    [Google Scholar]
  23. Sterne M., van Heyningen W. E. 1958; Clostridia. 545–575 Dubos R. J., Hirsh J. G. Bacterial and mycotic infections of man, 4. Lippincott Co; Philadelphia:
    [Google Scholar]
  24. Tissier H. 1918; La bacille de Barat. C. R. Soc. Biol Paris; 81426–427
    [Google Scholar]
  25. van Heyningen W. E. 1941; The biochemistry of the gangrene toxins. I. Estimation of the alpha-antitoxin of Cl. welchii type A. Biochem. J 35:1246–1256
    [Google Scholar]
  26. Willis A. T., Hobbs G. 1958; A medium for the identification of clostridia producing opales-cence in egg yolk emulsion. J. Pathol. Bacteriol 75:299–305
    [Google Scholar]
  27. Wirtz K. 1908; Eine einfache Art der Sporen-färbung. Zentralbl. Bakteriol. Parasitenk. Infek-tionskr. Hyg Abt. Orig 46:727–728
    [Google Scholar]
  28. Yamagishi T., Ishida S., Nishida S. 1964; Isolation of toxigenic strains of Clostridium perfringens. J. Bacteriol 88:646–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-23-4-419
Loading
/content/journal/ijsem/10.1099/00207713-23-4-419
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error