Classification of the microbacteria in a separate genus, Orla-Jensen, has been rejected by some authors. In this study three strains of Orla-Jensen, the type species of the genus, one strain of Orla-Jensen, and two strains of McLean and Sulzbacher were examined with the hope that the somewhat precarious taxonomic position of these species might be clarified. Included in this examination were: The determination of deoxyribonucleic acid (DNA) base composition by the thermal denaturation technique, the development of protein and esterase profiles by polyacrylamide gel electrophoresis, and selected enzyme assays associated with the Embden-Meyerhof, hexosemono-phosphate, and tricarboxylic acid pathways. The DNA base ratio studies showed to have a per cent guanine plus cytosine content of 36 compared with 58 for and 64 for The enzyme assays also indicated heterogeneity. All possessed enzymes associated with the Embden-Meyerhof and the hexosemonophosphate pathways, but alone failed to yield enzymatic evidence for an operational tricarboxylic acid cycle. Also, only showed evidence of a glyoxylate shunt. Protein profiles and esterase patterns were also sufficiently different to suggest a separation of from and These studies furnish additional evidence that be placed in the genus Lehmann and Neumann 1896. is an unusual organism which does not appear to be assignable to any of the presently recognized families of bacteria. The placement of in the genus Lehmann and Neumann 1896 would render Orla-Jensen 1919 a later, subjective synonym of does possess characteristics not unlike those of the plant pathogenic corynebacteria.


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