1887

Abstract

The extracytoplasmic function sigma factor, , has been shown to play a critical role in virulence of serovar Typhimurium ( Typhimurium). The previously optimized two-plasmid system has been used to identify Typhimurium promoters recognized by RNA polymerase containing . This method allowed identification of 34 -dependent promoters that direct expression of 62 genes in Typhimurium, 23 of which (including several specific for Typhimurium) have not been identified previously to be dependent upon in . The promoters were confirmed in Typhimurium and transcriptional start points of the promoters were determined by S1-nuclease mapping. All the promoters contained sequences highly similar to the consensus sequence of -dependent promoters. The identified genes belonging to the Typhimurium -regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and inner- and outer-membrane proteins with unknown functions. Several of these -dependent genes have been shown to play a role in virulence of Typhimurium.

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2006-05-01
2019-10-23
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