@article{mbs:/content/journal/micro/10.1099/mic.0.28744-0, author = "Skovierova, Henrieta and Rowley, Gary and Rezuchova, Bronislava and Homerova, Dagmar and Lewis, Claire and Roberts, Mark and Kormanec, Jan", title = "Identification of the σE regulon of Salmonella enterica serovar Typhimurium", journal= "Microbiology", year = "2006", volume = "152", number = "5", pages = "1347-1359", doi = "https://doi.org/10.1099/mic.0.28744-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28744-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "EσE, RNA polymerase holoenzyme containing σE", keywords = "OPP, oligopeptide permease", keywords = "ESR, extracytoplasmic stress response", keywords = "OPG, osmoregulated periplasmic glucan(s)", keywords = "TSP, transcription start point", keywords = "ECF, extracytoplasmic function", abstract = "The extracytoplasmic function sigma factor, σ E, has been shown to play a critical role in virulence of Salmonella enterica serovar Typhimurium (S. Typhimurium). The previously optimized two-plasmid system has been used to identify S. Typhimurium promoters recognized by RNA polymerase containing σ E. This method allowed identification of 34 σ E-dependent promoters that direct expression of 62 genes in S. Typhimurium, 23 of which (including several specific for S. Typhimurium) have not been identified previously to be dependent upon σ E in Escherichia coli. The promoters were confirmed in S. Typhimurium and transcriptional start points of the promoters were determined by S1-nuclease mapping. All the promoters contained sequences highly similar to the consensus sequence of σ E-dependent promoters. The identified genes belonging to the S. Typhimurium σ E-regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and inner- and outer-membrane proteins with unknown functions. Several of these σ E-dependent genes have been shown to play a role in virulence of S. Typhimurium.", }