1887

Abstract

serovar Enteritidis has emerged during the last 20 years as the major causative agent of food-borne gastroenteritis in humans and as the major infectious agent on poultry farms, replacing serovar Typhimurium as the dominant pathogenic serovar. Because adhesion to gut tissues and colonization of the alimentary tract, mediated in large part by the FimH adhesins located on type 1 fimbriae, is an important stage in the pathogenesis of both serovars, the binding properties of the FimH adhesins from these two enteropathogens were compared. Enteritidis FimH protein and the Typhimurium low-adhesive variant of this adhesin were expressed in and the recombinant proteins were analysed for their ability to bind glycoproteins carrying different oligomannosidic structures and different types of eukaryotic cells. In static binding assays (ELISA and Western blotting) both FimH proteins bound equally well to all three tested glycoproteins (RNase B, horseradish peroxidase and mannan-BSA). In addition, no differences were found in the binding specificity of the FimH proteins and intact cells of Enteritidis and Typhimurium to human colon carcinoma or bladder cancer cells. The presence of the same amino acid residues at positions 61 (glycine) and 118 (phenylalanine) and the similar binding properties of these two adhesins suggest that the newly described FimH protein of Enteritidis represents the low-adhesive variant found in Typhimurium. To study the binding specificity of Enteritidis FimH protein further, direct kinetic analysis using surface plasmon resonance was performed. With this method it was found that Enteritidis FimH adhesin bound with the highest value to high-mannose type -glycans carried by RNase B; about 100 times lower values were obtained in the interactions with mannan-BSA and horseradish peroxidase.

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2006-05-01
2019-11-14
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