1887

Abstract

The lipid-rich , to which and species belong, produce both fatty acids and mycolic acids. Compared with most other bacteria, possesses two fatty acid synthases, encoded by (8907 kb; FAS-IA) and (8988 kb; FAS-IB). Here, it was shown by mutational analyses that is essential but is not. However, in a background, the mutation results in a slightly reduced growth yield, -glutamate production is increased, and comparative lipid analysis suggests that FAS-IB is active primarily to supply palmitate. Transcript quantifications revealed that the transcript contributes 32 % to both transcripts during growth on glucose, affirmative for expression, and that is subordinate to . The transcript is downregulated by 8·3-fold during growth on acetate as compared with glucose. The lipid analyses also demonstrate that cells grown on propionate produce a number of uneven fatty acids (e.g. 15 : 0, 17 : 0, 17 : 1), which are not present in cells grown on glucose or acetate, suggesting that fatty acid synthase may also use propionyl-CoA as the priming unit in fatty acid synthesis. The fatty acid auxotrophic double mutant was used to determine the suggested incorporation of fatty acids into mycolic acids. Supplementation of this mutant with uniformly labelled [C]oleate and analysis of isolated mycolic acids confirmed that mature mycolic acids in the mutant consist exclusively of two fused [C]oleate molecules. In addition to an altered phospholipid profile, the mutant also exhibits differences in its mycolic acid profile. Taken together, the results show that although FAS-IA is the most relevant fatty acid synthase of and FAS-IB is supplementary, both synthases are necessary to produce the characteristic lipid environment of this organism.

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2005-07-01
2019-10-14
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