1887

Abstract

The presence of a polar species of glycopeptidolipid (GPL) in carbon-starved has been reported previously. In this study, the complete structure of this GPL is established with the help of MALDI-TOF (matrix assisted laser desorption/ionization time of flight) and ESI (electrospray ionization) -MS, C-SEFT (spin echo Fourier transform) -NMR spectroscopy, and HPLC analysis. In the molecule, two units of a 3,4-di--methyl derivative of rhamnose are attached to -alaninol via a 1→2 linkage. Various methyl derivatives of rhamnose and 6-deoxytalose were synthesized as standards to establish this structure. The accumulation of this polar GPL in is dependent, as a SigB-overproducing strain of shows the presence of this spot in the exponential phase, and a -knockout strain of does not show the presence of any polar GPLs.

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2005-07-01
2020-08-06
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