1887

Abstract

It has been demonstrated that plasmids are not randomly distributed but are located symmetrically in mid-cell, or ¼, ¾ positions in bacterial cells. In this work we compared the localization of broad-host-range plasmid RK2 mini-replicons, which lack an active partitioning system, in and cells. In the location of the plasmid mini-replicon cluster was at the cell poles. In contrast, in cells, as a result of the interaction of chromosomally encoded ParB protein with RK2 centromere-like sequences, these mini-derivatives were localized in the proximity of mid-cell, or ¼, ¾ positions. The expression of the genes in resulted in a positional change in the RK2 mini-derivative to the mid-cell or ¼, ¾ positions. Moreover, in a mutant, both RK2 mini-derivatives and the entire RK2 plasmid exhibited disturbances of subcellular localization. These observations raise the possibility that in certain bacteria chromosomally encoded partitioning machinery could affect subcellular plasmid positioning.

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2008-09-01
2020-08-15
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