@article{mbs:/content/journal/micro/10.1099/mic.0.2008/018762-0, author = "Kolatka, Katarzyna and Witosinska, Monika and Pierechod, Marcin and Konieczny, Igor", title = "Bacterial partitioning proteins affect the subcellular location of broad-host-range plasmid RK2", journal= "Microbiology", year = "2008", volume = "154", number = "9", pages = "2847-2856", doi = "https://doi.org/10.1099/mic.0.2008/018762-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2008/018762-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "FISH, fluorescence in situ hybridization", keywords = "DAPI, 4′,6-diamidino-2-phenylindole", abstract = "It has been demonstrated that plasmids are not randomly distributed but are located symmetrically in mid-cell, or ¼, ¾ positions in bacterial cells. In this work we compared the localization of broad-host-range plasmid RK2 mini-replicons, which lack an active partitioning system, in Escherichia coli and Pseudomonas putida cells. In E. coli the location of the plasmid mini-replicon cluster was at the cell poles. In contrast, in Pseudomonas cells, as a result of the interaction of chromosomally encoded ParB protein with RK2 centromere-like sequences, these mini-derivatives were localized in the proximity of mid-cell, or ¼, ¾ positions. The expression of the Pseudomonas parAB genes in E. coli resulted in a positional change in the RK2 mini-derivative to the mid-cell or ¼, ¾ positions. Moreover, in a P. putida parAB mutant, both RK2 mini-derivatives and the entire RK2 plasmid exhibited disturbances of subcellular localization. These observations raise the possibility that in certain bacteria chromosomally encoded partitioning machinery could affect subcellular plasmid positioning.", }