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Abstract

Genes encoding a novel multidrug efflux pump, AadT, from the Drug:H antiporter 2 family, were discovered in multidrug resistance plasmids. Here, we profiled the antimicrobial resistance potential, and examined the distribution of these genes. homologs were found in many and other Gram-negative species and were typically adjacent to novel variants of ), which encodes a major tripartite efflux pump in . The AadT pump decreased bacterial susceptibility to at least eight diverse antimicrobials, including antibiotics (erythromycin and tetracycline), biocides (chlorhexidine), and dyes (ethidium bromide and DAPI) and was able to mediate ethidium transport. These results show that AadT is a multidrug efflux pump in the resistance arsenal and may cooperate with variants of AdeAB(C).

Funding
This study was supported by the:
  • National Health and Medical Research Council (Award GNT1120298)
    • Principle Award Recipient: KarlA. Hassan
  • Australian Research Council (Award FT180100123)
    • Principle Award Recipient: KarlA. Hassan
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/micro/10.1099/mic.0.001341
2023-05-30
2024-05-03
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