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Volume 166, Issue 2

Adaptive laboratory evolution of under acid stress Free

Abstract

The ability of to tolerate acid stress is important for its survival and colonization in the human digestive tract. Here, we performed adaptive laboratory evolution of the laboratory strain K-12 MG1655 at pH 5.5 in glucose minimal medium. After 800 generations, six independent populations under evolution had reached 18.0 % higher growth rates than their starting strain at pH 5.5, while maintaining comparable growth rates to the starting strain at pH 7. We characterized the evolved strains and found that: (1) whole genome sequencing of isolated clones from each evolved population revealed mutations in appearing in five of six sequenced clones; and (2) gene expression profiles revealed different strategies to mitigate acid stress, which are related to amino acid metabolism and energy production and conversion. Thus, a combination of adaptive laboratory evolution, genome resequencing and expression profiling revealed, on a genome scale, the strategies that uses to mitigate acid stress.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acid stress , adaptive laboratory evolution , Escherichia coli , RNA sequencing and whole genome resequencing
Funding
This study was supported by the:
  • Novo Nordisk Fonden (Award NNF10CC1016517)
    • Principle Award Recipient: Bernhard O. Palsson
  • National Institute of General Medical Sciences (Award R01GM057089)
    • Principle Award Recipient: Bernhard O. Palsson
© 2020 The Authors
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2019-10-18
2024-05-04
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Adaptive laboratory evolution of Escherichia coli under acid stress
Microbiology 166, 141 (2020); https://doi.org/10.1099/mic.0.000867
/content/journal/micro/10.1099/mic.0.000867
/content/journal/micro/10.1099/mic.0.000867
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