1887

Abstract

SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I More than 30 × 10organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.

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/content/journal/micro/10.1099/00221287-63-2-211
1970-10-01
2021-07-24
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