%0 Journal Article %A Stainer, D. W. %A Scholte, M. J. %T A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis %D 1970 %J Microbiology, %V 63 %N 2 %P 211-220 %@ 1465-2080 %R https://doi.org/10.1099/00221287-63-2-211 %I Microbiology Society, %X SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 × 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-63-2-211