1887

Abstract

The dsRNA viruses of the corn pathogen encode toxins that affect sensitive strains of the same species and related species. The KP6 toxin encoded by the P6 virus subtype is a binary toxin, consisting of two polypeptides, α (8-6 kDa) and β (9-1 kDa) with no covalent or hydrogen bonds between them. In this study the effect of each polypeptide was tested on sensitive and resistant cells and spheroplasts of . The results indicate that both polypeptides bind to intact cells from sensitive and resistant strains; however, only spheroplasts of sensitive strains are affected by the toxin and both α and β are necessary to affect the spheroplasts.

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1994-03-01
2021-10-20
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References

  1. Al-Aidroos K., Bussey H. Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can J Microbiol 1978; 24:228–237
    [Google Scholar]
  2. Boone C., Sommer S., Hensel A., Bussey H. Yeast KRE genes provide evidence for a pathway of cell wall a-glucan assembly. J Cell Biol 1990; 110:1833–1843
    [Google Scholar]
  3. Bostian K. A., Elliott Q., Bussey H., Burn V., Smith A., Tipper D. Sequence of the pretoxin dsRNA gene of type I killer yeast: multiple processing events produce a two component toxin. Cell 1984; 36:741–751
    [Google Scholar]
  4. Buck K. W. Fungal virology - an overview. In Fungal Virology 1986 Edited by Buck K. W. Boca Raton: CRC Press; pp 1–84
    [Google Scholar]
  5. Bussey H. Kl killer toxin, a pore forming protein from yeast. Mol Microbiol 1991; 5:2339–2343
    [Google Scholar]
  6. Bussey H., Skipper N. Membrane mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata . J Bacteriol 1975; 124:476–483
    [Google Scholar]
  7. Butler A. R., Porter M., Stark M. J. R. Intracellular expression of Kluyveromyces lactis toxin γ subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutants. Yeast 1991; 7:617–625
    [Google Scholar]
  8. Finkler A., Peery T., Tao J., Bruenn J., Koltin Y. Immunity and resistance to the KP6 toxin of Ustilago maydis . Mol & Gen Genet 1992; 233:395–403
    [Google Scholar]
  9. Ginzberg I. 1990 The Ustilago maydis killer system: the organisation of the toxin encoding genes and characterisation of their productsPhD thesis Tel Aviv University
    [Google Scholar]
  10. Holliday R. The genetics of Ustilago maydis . Genet Res 1961; 2:204–230
    [Google Scholar]
  11. Hutchins K., Bussey H. Cell wall receptor for yeast killer toxin: involvement of (l-6)-β-d-glucan. J Bacteriol 1983; 154:161–169
    [Google Scholar]
  12. Kagan B. L. Mode of action of yeast killer toxins: channel formation in lipid bilayer membranes. Mature 1983; 302:709–711
    [Google Scholar]
  13. Koltin Y. The killer system of Ustilago maydis:secreted polypeptides encoded by viruses . In Viruses of Fungi and Simple Fukaryotes 1988 Edited by Koltin Y., Leibowitz M. New York: Marcel Dekker; pp 209–242
    [Google Scholar]
  14. Koltin Y., Kandel J. S. Killer phenomenon in Ustilago maydis: the organization of the viral genome. Genetics 1978; 88:267–276
    [Google Scholar]
  15. Kopecky A. L., Copeland D. P., Lusk J. E. Viability of Escherichia coli treated with colicin K. Proc Natl Acad Sci USA 1975; 72:4631–4634
    [Google Scholar]
  16. Levine R., Koltin Y., Kandel J. Nuclease activity is associated with the Ustilago maydis virus induced killer proteins. Nucleic Acids Res 1979; 6:3717–3731
    [Google Scholar]
  17. Martinac B., Zhu H., Kubalski A., Zhou X., Culbertson M., Bussey H., Kung C. Yeast kl killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Proc Natl Acad Sci USA 1990; 87:6228–6232
    [Google Scholar]
  18. Meaden P., Hill K., Wagner J., Slipetz D., Sommer S. S., Bussey H. The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (l-6)-β-d-glucan synthesis and normal cell growth. Mol Cell Biol 1990; 10:3013–3019
    [Google Scholar]
  19. Middelbeek E. J., van de Lar H. H. A. M., Hermans J. M. H., Stumm C., Vogels G. D. Physiological conditions affecting the sensitivity of Saccharomyces cerevisiae to a Pichia kluyveri killer toxin and energy requirements for toxin action. Antonie Eeeuwenhoek 1980; 46:483–497
    [Google Scholar]
  20. Peery T. 1988 Ustilago maydis virus encoded toxin: purification and characterisationPhD thesis Tel Aviv University
    [Google Scholar]
  21. Peery T., Shabat-Brand T., Steinlauf R., Koltin Y., Bruenn J. Virus-encoded toxin of Ustilago maydis: Two polypeptides are essential for activity. Mol Cell Biol 1987; 7:470–477
    [Google Scholar]
  22. Schmitt M., Radler F. Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of Saccharomyces cerevisiae strain 28. J Gen Microbiol 1987; 133:3347–3354
    [Google Scholar]
  23. Sherman F., Fink G. R., Hicks J. B. Methods in Yeast Genetics 1982 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Stark M. J., R., Boyd A., Mileham A. J., Romanos M. A. The plasmid encoded killer system of Kluyveromyces lactis: a review. Yeast 1990; 6:1–29
    [Google Scholar]
  25. Steinlauf R., Peery T., Koltin Y., Bruenn J. The Ustilago maydis virus-encoded toxin: effect of KP6 on sensitive cells and spheroplasts. Exp Mycol 1988; 12:264–274
    [Google Scholar]
  26. Sturley S. L., Elliot Q., LeVitre J., Tipper D. J., Bostian K. A. Mapping of functional domains within the Saccharomyces cerevisiae type 1 killer preprotoxin. EMBO J 1986; 5:3381–3389
    [Google Scholar]
  27. Tao J., Ginzberg I., Banerjee N., Held W., Koltin Y., Bruenn J. Ustilago maydis KP6 killer toxin: structure, expression in Saccharomyces cerevisiae, and relationship to other cellular toxins. Mol Cell Biol 1990; 10:1373–1381
    [Google Scholar]
  28. Tipper D. J., Schmitt M. J. Yeast dsRNA viruses: replication and killer phenotypes. Mol Microbiol 1991; 5:2331–2338
    [Google Scholar]
  29. Wessels J. G. H. Morphogenesis and biochemical processes in Schispphyllum commune FR. Wentia 1965; 13:1–113
    [Google Scholar]
  30. Wickner R. B. Double-stranded RNA replication in yeast: the killer system. Annu Rev Biochem 1986; 55:373–395
    [Google Scholar]
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