%0 Journal Article %A Ginzberg, Idit %A Koltin, Yigal %T Interaction of the Ustilago maydis KP6 killer toxin with sensitive cells %D 1994 %J Microbiology, %V 140 %N 3 %P 643-649 %@ 1465-2080 %R https://doi.org/10.1099/00221287-140-3-643 %K killer toxins %K Ustilago maydis %K d5RNA virus toxins %I Microbiology Society, %X The dsRNA viruses of the corn pathogen Ustilago maydis encode toxins that affect sensitive strains of the same species and related species. The KP6 toxin encoded by the P6 virus subtype is a binary toxin, consisting of two polypeptides, α (8-6 kDa) and β (9-1 kDa) with no covalent or hydrogen bonds between them. In this study the effect of each polypeptide was tested on sensitive and resistant cells and spheroplasts of U. maydis. The results indicate that both polypeptides bind to intact cells from sensitive and resistant strains; however, only spheroplasts of sensitive strains are affected by the toxin and both α and β are necessary to affect the spheroplasts. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-3-643