1887

Abstract

Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the GS-5 genome using restriction enzymes I (5′-GGGCC/C), I (5′-CCC/GGG), and I (5′-GC/GGCCGC).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-1-67
1993-01-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/1/mic-139-1-67.html?itemId=/content/journal/micro/10.1099/00221287-139-1-67&mimeType=html&fmt=ahah

References

  1. Aduse-Opoku J, Tao L., Ferretti J.J, Russell R.R.B. 1991; Biochemical and genetic analysis of Streptococcus mutans α- galactosidase. Journal of General Microbiology 137:757–764
    [Google Scholar]
  2. Amjad M., Castro J.M, Sandoval H., Wu J.J, Henner D.J, Piggot P.J. 1990; An Sfi restriction map of the Bacillus subtilis 168 genome. Gene 101:15–21
    [Google Scholar]
  3. Aoki H., Shiroza T., Hayakawa M., Sato S., Kuramitsu H.K. 1986; Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infection and Immunity 53:587–594
    [Google Scholar]
  4. Banas J.A., Russell R.R.B, Ferretti J.J. 1990; Sequence analysis of the gene for the glucan-binding protein of Streptococcus mutans Ingbritt. Infection and Immunity 58:667–673
    [Google Scholar]
  5. Brady L.J., Crowley P.J, Ferretti J.J, Ma J.K.C., Kelly C., Lee S.F, Lehner T., Bleiweis A.S. 1991; Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates. Infection and Immunity 59:1803–1810
    [Google Scholar]
  6. Bratthall D. 1970; Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans.. Odontologisk Revy 21:143–152
    [Google Scholar]
  7. Burne R.A, Rubinfeld B., Bowen W.H, Yasbin .R.E. 1986; Tight genetic linkage of a glucosyltransferase and dextranase of Streptococcus mutans GS−5. Journal of Dental Research 65:1392–1401
    [Google Scholar]
  8. Carle G.F, Olson M.V. 1984; Separation of chromosomal DNA molecules from yeast by orthogonal field alternation gel electrophoresis . Nucleic Acids Research 12:5647–5664
    [Google Scholar]
  9. Caufield P.W, Walker T.M. 1989; Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. Journal of Clinical Microbiology 27:274–278
    [Google Scholar]
  10. Chang N., Taylor D.E. 1990; Use of pulsed field agarose gel electrophoresis to size genomes of Campylobacter jejuni UA580. Journal of Bacteriology 172:5211–5217
    [Google Scholar]
  11. Chia J.S, Hsu T.Y, Teng L.J, Chen J.Y, Hahn L.J, Yang C.S. 1991; Glucosyltransferase gene polymorphism among Streptococcus mutans strains. Infection and Immunity 59:1656–1660
    [Google Scholar]
  12. Chu G., Vollrath D., Davis R.W. 1986; Separation of large DNA molecules by contour-clamped homogenous electric fields . Science 234:1582–1585
    [Google Scholar]
  13. Cook P.R. 1984; A general method for preparing intact nuclear DNA. EM BO Journal1837–1842
    [Google Scholar]
  14. Coykendall A.L. 1974; Four types of Streptococcus mutans based on their genetic, antigenic, and biochemical characteristics. Journal of General Microbiology 83:327–338
    [Google Scholar]
  15. Feinberg A.P, Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  16. Ferretti J.J, Russell R.R.B, Dao M.L. 1989; Sequence analysis of the wall-associated protein precursor of Streptococcus mutans antigen. A Molecular Microbiology 3:469–478
    [Google Scholar]
  17. Gasc A.M, Kauc L., Barraille P., Sicard M., Goodgal S.H. 1991; Gene localization, size, and physical map of the chromosome of Streptococcus pneumoniae. Journal of Bacteriology 173:7361–7367
    [Google Scholar]
  18. Gawron Burke C., Clewell D.B. 1984; Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichiacoli:strategy for targeting and cloning of genes from gram-positivebacteria. Journal of Bacteriology 159:214–221
    [Google Scholar]
  19. Hanada N., Kuramitsu H.K. 1988; Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infection and Immunity 56:1999–2005
    [Google Scholar]
  20. Hanada N., Kuramitsu H.K. 1989; Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer dependent soluble glucan synthesis. Infection and Immunity 57:2079–2085
    [Google Scholar]
  21. Hantman M.J, Tudor J.J, Sun S., Marri L., Piggot P.J., Daneo Moore L. 1991; Physical and genetic mapping of the Streptococcus mutans GS−5 genome. In Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci p. 289, Edited by . Dunny G.M.P, Cleary P., McKay L.L. Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  22. Hayakawa M., Aoki. H. 1986; Isolation and characterization of the sucrose 6-phosphate hydrolase gene from Streptococcus mutans. Infection and Immunity 53:582–586
    [Google Scholar]
  23. Hillman J.D, Duncan M.J, Stashenko K.P. 1990; Cloning and expression of the gene encoding the fructose-1,6-diphosphate- dependent L-( + )-lactate dehydrogenase of Streptococcus mutans. Infection and Immunity 58:1290–1295
    [Google Scholar]
  24. Itaya M., Tanaka T. 1991; Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. Journal of Molecular Biology 220:631–648
    [Google Scholar]
  25. Krynicka Jagusztyn E.K, Smorawinska M., Curtiss R.III. 1982; Expression ofStreptococcus mutans aspartate-semialdehyde dehydrogenase gene cloned into plasmid pBR322. Journal of General Microbiology 128:1135–1145
    [Google Scholar]
  26. Krause D.C., Mawn C.B. 1990; Physical analysis and mapping of the Mycoplasma pneumoniae chromosome. Journal of Bacteriology 172:4790–4797
    [Google Scholar]
  27. Lebourgeois P., Mata M., Ritzenthaler P. 1989; Genome comparison of Lactococcus strains by pulsed field gel electrophoresis. FEMS Microbiology Letters 59:65–70
    [Google Scholar]
  28. Lee S.F., Progulske-Fox A., Bleiweis A.S. 1988; Molecular cloning and expression of a Streptococcus mutans erichia coli. Infection and Immunity 56:2114–2119
    [Google Scholar]
  29. Loesche W.J. 1986; Role of Streptococcus mutans in human dental decay. Microbiolog. Rev 50:353–380
    [Google Scholar]
  30. Lunsford R.D., Macrina F.L. 1986; Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoeno/pyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase. Journal of Bacteriology 166:426–434
    [Google Scholar]
  31. Mathew M.K, Smith C.L., Cantor C.R. 1988; High- resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperature. Biochemistry 27:9204–9210
    [Google Scholar]
  32. Morrissey P., Dougan G., Russell R.R.B, Gilpin M. 1985; Cloning of Streptococcus mutans antigens in Escherichia coli as an aid to the development of a dental caries vaccine. In Vaccines 85, pp. 117–120, Edited by. Lerner R.A., Chanock R.M., Brown F. Spring Harbor, NY: Cold Spring Harbor Laboratory Press:
    [Google Scholar]
  33. Okahashi N., Sasakawa C., Yoshikawa M., Hamada S., Koga T. 1989; Cloning of a surface protein antigen gene from serotype c Streptococcus mutans. Molecular Microbiology 3:221–228
    [Google Scholar]
  34. Okahashi N., Sasakawa C., Okada N., Yamada M., Tokuda M., Takahashi I., Koga T. 1990; Construction of a Not\restriction map of the Streptococcus mutans genome. Journal of General Microbiology 136:2217–2223
    [Google Scholar]
  35. Perry D., Kuramitsu H.K. 1989; Genetic linkage among cloned genes of Streptococcus mutans. Infection and Immunity 57:805–809
    [Google Scholar]
  36. Perry D., Kuramitsu H.K. 1990; Linkage of sucrose metabolizing genes in Streptococcus mutans. Infection and Immunity 58:3462–3464
    [Google Scholar]
  37. Perry D., Nelson L.J., Kuramitsu H.K. 1985; Mapping of a cloned glucosyltransferase gene in Streptococcus mutans. Infection and Immunity 50:130–135
    [Google Scholar]
  38. Piggot P.J, Curtis C.A.M. 1987; Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions. Journal of Bacteriology 169:1260–1266
    [Google Scholar]
  39. Procino J.K., Marri L., Shockman G.D., Daneo Moore L. 1988; Tn916 insertional inactivation of multiple genes on the chromosome of Streptococcus mutans GS−5. Infection and Immunity 56:2866–2870
    [Google Scholar]
  40. Pucci M.J., Macrina F.L. 1986; Molecular organization and expression of the gtfA gene of Streptococcus mutans LM7. Infection and Immunity 54:77–84
    [Google Scholar]
  41. Reider J.L, Macrina F.L. 1976; Plasmid DNA isolation in Streptococcus mutans : glycine enhanced cell lysis. In Proceedings: Microbial Aspects of Dental Caries (a special supplement to Microbiology, abstracts) 3 pp. 725–736, Edited by. Stile H. M., Loesche W.J., 'Brien Loesche T.C.O, 'Brien T.C.O. Washington, DC:: Information Retrieval.;
    [Google Scholar]
  42. Robeson J.P, Barletta R.G., Curtiss R.III. 1983; Expression of a Streptococcus mutans glucosyltransferase gene in Escherichia coli. Journal of Bacteriology 153:211–221
    [Google Scholar]
  43. R Russell. R., J Ferretti. J. 1990; Nucleotide sequence of the dextran glucosidase (dexB) gene of Streptococcus mutans. Journal of General Microbiology 136:803–810
    [Google Scholar]
  44. Russell R.R.B., Coleman D., Dougan G. 1985; Expression of a gene for glucan-binding protein from Streptococcus mutans. in Escherichia coli. Journal of General Microbiology 131:295–299
    [Google Scholar]
  45. Russell R.R.B., Aduse Opoku J., Tao L., Ferretti J.J. 1991; Binding protein-dependent transport system in Streptococcus mutans. In Genetics and Molecular Biology of Streptococci Lactococci, and Enterococci, pp. 244–247, Edited by. Dunny G. M., Cleary P. P., McKay L. L., Ferretti J.J. Washington, DC:: American Society for Microbiology:
    [Google Scholar]
  46. Sato S., Kuramitsu H.K. 1986; Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS−5. Infection and Immunity 52:166–170
    [Google Scholar]
  47. Sato Y., Poy F., Jacobson G.R., Kuramitsu H.K.. 1989; Characterization and sequence analysis of the scrA gene encoding enzyme IIScr of the Streptococcus mutans phosphocno/pyruvate- dependent sucrose phosphotransferase system. Journal of Bacteri-ology 171:263–271
    [Google Scholar]
  48. Schwartz D.C, Cantor C.R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
    [Google Scholar]
  49. Shiroza T., Kuramitsu H.K. 1988; Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions. Journal of Bacteriology 170:810–816
    [Google Scholar]
  50. Shiroza T., Ueda S., Kuramitsu H.K. 1987; Sequence analysis of the gtfB gene from Streptococcus mutans. Journal of Bacteriology 169:4263–1270
    [Google Scholar]
  51. Smith C.L., Cantor C.R. 1987; Purification, specific fragmentation, and separation of large DNA molecules. Methods in Enzymology 155:449–467
    [Google Scholar]
  52. Smith C., Econome L., Schutt G., Klco A., Cantor C.F. 1987; A physical map of the Escherichia coli K12 genome . Science 235:1448–1453
    [Google Scholar]
  53. Smorawinska M., Hsu J.C., Hansen J.B., Jagusztyn-Kry E.K., Abiko Y., Curtiss R.III. 1983; Clustered genes for galactose metabolism from Streptococcus cloned in E. coli. Journal of Bacteriology 153:1095–1097
    [Google Scholar]
  54. Southern E.M. 1975; Detection of sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–507
    [Google Scholar]
  55. Tanskanen E.I., Tulloch D.L., Hillier A.J., Davidson B.E. 1990; Pulsed field gel electrophoresis of Smal digests of lactococcal genomic DNA, a novel method of strain identification. Applied and Environmental Microbiology 56:3105–3111
    [Google Scholar]
  56. Tudor J.J., Marri L., Piggot P.J., Daneo Moore L. 1990; Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed field gel electrophoresis. Infection and Immunity 58:838–840
    [Google Scholar]
  57. Ueda S., Shiroza T., Kuramitsu H.K. 1988; Sequence analysis of the gtfC gene from Streptococcus mutans GS−5. Gene 69:101–109
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-1-67
Loading
/content/journal/micro/10.1099/00221287-139-1-67
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error