1887

Abstract

Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the GS-5 genome using restriction enzymes I (5'-GGGCC/C), I (5'-CCC/GGG), and I (5'-GC/GGCCGC).

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/content/journal/micro/10.1099/00221287-139-1-67
1993-01-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-1-67
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