RT Journal Article SR Electronic(1) A1 Hantman, Michael J. A1 Sun, Shuzhong A1 Piggot, Patrick J. A1 Daneo-Moore, LolitaYR 1993 T1 Chromosome organization of Streptococcus mutans GS-5 JF Microbiology, VO 139 IS 1 SP 67 OP 77 DO https://doi.org/10.1099/00221287-139-1-67 PB Microbiology Society, SN 1465-2080, AB Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5′-GGGCC/C), SmaI (5′-CCC/GGG), and NotI (5′-GC/GGCCGC)., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-1-67