SUMMARY: We have selected for genes that, when present in multiple copies, enhance growth of wild-type cells of in an iron-limiting medium. A gene designated , for ‘ferric utilization proficient’, was isolated by this approach. Increased dosage of reduces the concentration of iron in the medium required for efficient growth and confers elevated levels of iron uptake activity in iron-limited cells. Disruption of the locus reduces wild-type iron uptake rates by 2-fold in cells grown on raffinose medium but has no effect on glucose-grown cells. DNA sequencing showed that encodes a hydrophilic 43 kDa protein identical to , a gene encoding a transcriptional activator implicated in carbon source regulation. Our results suggest that also regulates synthesis of gene products involved in iron uptake.


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