1887

Abstract

SUMMARY: Previously we reported [Deane, S. M., Maharaj, R., Robb, F. T. & Woods, D. R. (1987) 133, 2295-2302] that the production of a SDS-resistant alkaline serine protease (Pro A) cloned in was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active Pro A. Replacement of the promoter region by the -amylase promoter region from resulted in the simultaneous synthesis and secretion of Pro A in The gene cloned on a shuttle vector did not produce active Pro A in . Although Pro A has a typical Gram-positive signal sequence, it was not functional in Replacement of the Pro A signal sequence with the -amylase signal sequence resulted in the production of active Pro A in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-2-319
1992-02-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/2/mic-138-2-319.html?itemId=/content/journal/micro/10.1099/00221287-138-2-319&mimeType=html&fmt=ahah

References

  1. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Forrest F., Magasanik B. 1977; Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. Journal of Bacteriology 129:1001–1009
    [Google Scholar]
  2. Calogero S., Albertini A. M., Fogher C, Marzari R., Galizzi A. 1989; Expression of a cloned Bacillus thuringiensis delta-endotoxin gene in Bacillus subtilis. Applied and Environmental Microbiology 55:446–453
    [Google Scholar]
  3. Corfield V. A., Reid S. J., Bodmer J., Thomson J. A. 1984; A modified protoplast-regeneration protocol facilitating the detection of cloned exoenzyme genes in Bacillus subtilis. Gene 30:17–22
    [Google Scholar]
  4. Dagert M., Ehrlich S. D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  5. Deane S. M., Robb F. T., Woods D. R. 1986; Isolation and characterization of a Vibrio alginolyticus mutant that overproduces extracellular proteases. Journal of General Microbiology 132:893–898
    [Google Scholar]
  6. Deane S. M., Robb F. T., Woods D. R. 1987a; Production and activation of an SDS-resistant alkaline serine exoprotease of Vibrio alginolyticus. Journal of General Microbiology 133:391–398
    [Google Scholar]
  7. Deane S. M., Maharaj R., Robb F. T., Woods D. R. 1987b; Cloning, expression and release of a Vibrio alginolyticus SDS-resistant Ca2+-dependent exoprotease in Escherichia coli. Journal of General Microbiology 133:2295–2302
    [Google Scholar]
  8. Deane S. M., Robb F. T., Robb S. M., Woods D. R. 1989; Nucleotide sequence of the Vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease A. Gene 76:281–288
    [Google Scholar]
  9. Debaov V. G. 1982 The Molecular Biology of the Bacilli, Vol. I pp. 331–370 Edited by Dubnau D. A. London: Academic Press;
  10. Doi R. H., Wong S.-L., Kawamura F. 1986; Potential use of Bacillus subtilis for secretion and production of foreign proteins. TIBTECH September, 232–235
    [Google Scholar]
  11. Graves M. C., Rabinowitz J. C. 1986; In vivo and in vitro transcription of the Clostridium pasteurianum ferredoxin gene. Journal of Biological Chemistry 261:11409–11415
    [Google Scholar]
  12. Hare P., Long S., Robb F. T., Woods D. R. 1981; Regulation of exoprotease production by temperature and oxygen in Vibrio alginolyticus. Archives of Microbiology 130:276–280
    [Google Scholar]
  13. Hare P., Scottburden T., Woods D. R. 1983; Characterization of extracellular alkaline proteases and collagenase induction in Vibrio alginolyticus. Journal of General Microbiology 129:1141–1147
    [Google Scholar]
  14. von Heijne G. 1983; Patterns of amino acids near signal-sequence cleavage sites. European Journal of Biochemistry 133:17–21
    [Google Scholar]
  15. Ikemura H., Takagi H., Inouye M. 1987; Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. Journal of Biological Chemistry 262:7859–7864
    [Google Scholar]
  16. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  17. Joliff G., Edelman A., Klier A., Rapoport G. 1989; Inducible secretion of a cellulase from Clostridium thermocellum in Bacillus subtilis. Applied and Environmental Microbiology 55:2739–2744
    [Google Scholar]
  18. Kawamura F., Doi R. H. 1984; Construction of Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. Journal of Bacteriology 160:442–444
    [Google Scholar]
  19. Lin L.-L., Rumbak E., Zappe H., Thomson J. A., Woods D. R. 1990; Cloning, sequencing and analysis of expression of a Butyrivibrio fibrisolvens gene encoding a β-glucosidase. Journal of General Microbiology 136:1567–1576
    [Google Scholar]
  20. Long S., Mothibeli M. A., Robb F. T., Woods D. R. 1981; Regulation of extracellular alkaline protease activity by histidine in a collagenolytic Vibrio alginolyticus strain. Journal of General Microbiology 127:193–199
    [Google Scholar]
  21. Misra T. K. 1985; A new strategy to create ordered deletions for rapid nucleotide sequencing. Gene 34:263–268
    [Google Scholar]
  22. Overbeeke N., Termorshuizen G. H. M., Giuseppin M. L. F., Underwood D. R., Verrips C. T. 1990; Secretion of the a-galactosidase from Cyamopsis tetragonoloba (Guar) by Bacillus subtilis. Applied and Environmental Microbiology 56:1429–1434
    [Google Scholar]
  23. Palva I., Pettersson R. F., Kalkkinen N., Lehtovaara P., Sarvas M. S., Soderlund H., Takkinen K., Kaariainen L. 1981; Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the a-amylase gene from Bacillus amyloliquefaciens. Gene 15:43–51
    [Google Scholar]
  24. Petit M.-A., Joliff G., Mesas J. M., Klier A., Rapoport G., Ehrlich S. D. 1990; Hypersecretion of a cellulase from Clostridium thermocellum in Bacillus subtilis by induction of chromosomal DNA amplification. Bio/Technology 8:559–563
    [Google Scholar]
  25. Powers S. D., Adams R. M., Wells J. A. 1986; Secretion and autoproteolytic maturation of subtilisin. Proceedings of the National Academy of Sciences of the United States of America 833096–3100
  26. Reid G. C, Robb F. T., Woods D. R. 1978; Regulation of extracellular collagenase production in Achromobacter iophagus. Journal of General Microbiology 109:149–154
    [Google Scholar]
  27. Reid G. C, Woods D. R., Robb F. T. 1980; Peptone induction and rifampicin insensitive collagenase production by Vibrio alginolyticus. Journal of Bacteriology 142:447–454
    [Google Scholar]
  28. Rubenstein C. P., Moratinos L. C, COSO O. A., Sanchezrivas C. 1988; Improvements in the transformation of Bacillus subtilis protoplasts with plasmid DNA. FEMS Microbiology Letters 56:67–70
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
  30. Sarvas M. 1986; Protein secretion in Bacilli. Current Topics in Microbiology and Immunology 125:103–125
    [Google Scholar]
  31. Saunders C. W., Schmidt B. J., Mallonee R. L., Guyer M. S. 1987; Secretion of human serum albumin from Bacillus subtilis. Journal of Bacteriology 169:2917–2925
    [Google Scholar]
  32. Schein C. H., Kashiwagi K., Fujisawa A., Weissmann C. 1986; Secretion of mature IFN-α2 and accumulation of uncleaved precursor by Bacillus subtilis transformed with a hybrid a-amylase signal sequence-IFN-a2 gene. Bio/Technology 4:719–725
    [Google Scholar]
  33. Scholle R. R., Coyne V. E., Maharaj R., Robb F. T., Woods D. R. 1987; Expression and regulation of a Vibrio alginolyticus sucrose utilisation system cloned in Escherichia coli. Journal of Bacteriology 169:2685–2690
    [Google Scholar]
  34. Sykes R. B., Nordstrom K. 1972; Microiodometric determination of ^-lactamase activity. Antimicrobial Agents and Chemotherapy 1:94–99
    [Google Scholar]
  35. Vasantha N., Thompson L. D. 1986; Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis. Gene 49:23–28
    [Google Scholar]
  36. Wang L.-F., Wong S.-L., Lee S.-G., Kalyan N. K., Hung P. P., Hilliker S., Doi R. H. 1988; Expression and secretion of human atrial natriuretic a-factor in Bacillus subtilis using the subtilisin signal peptide. Gene 69:39–47
    [Google Scholar]
  37. Willis R. C, Morris R. G., Cirakoglu C, Schellenberg G. D., Gerber N. H., Furlong C. E. 1974; Preparation of the periplasmic binding proteins from Salmonella typhimurium and Escherichia coli. Archives of Biochemistry and Bicphysics 161:64–75
    [Google Scholar]
  38. Yanisch-Perron C, Vieira I., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  39. Zabeau M., Stanley K. K. 1982; Enhanced expression of cro-β-galactosidase fusion proteins under the control of the pR promoter of bacteriophage. EMBO Journal 1:1217–1224
    [Google Scholar]
/content/journal/micro/10.1099/00221287-138-2-319
Loading
/content/journal/micro/10.1099/00221287-138-2-319
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error