1887

Abstract

SUMMARY: Previously we reported [Deane, S. M., Maharaj, R., Robb, F. T. & Woods, D. R. (1987) 133, 2295-2302] that the production of a SDS-resistant alkaline serine protease (Pro A) cloned in was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active Pro A. Replacement of the promoter region by the -amylase promoter region from resulted in the simultaneous synthesis and secretion of Pro A in The gene cloned on a shuttle vector did not produce active Pro A in . Although Pro A has a typical Gram-positive signal sequence, it was not functional in Replacement of the Pro A signal sequence with the -amylase signal sequence resulted in the production of active Pro A in .

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1992-02-01
2021-05-14
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