%0 Journal Article %A Zappe, Harold %A Blatch, Gregory L. %A Woods, David R. %T Expression of Bacillus amyloliquefaciens amylase and Vibrio alginolyticus protease A fusion genes %D 1992 %J Microbiology, %V 138 %N 2 %P 319-327 %@ 1465-2080 %R https://doi.org/10.1099/00221287-138-2-319 %I Microbiology Society, %X SUMMARY: Previously we reported [Deane, S. M., Maharaj, R., Robb, F. T. & Woods, D. R. (1987) Journal of General Microbiology 133, 2295-2302] that the production of a Vibrio alginolyticus SDS-resistant alkaline serine protease (Pro A) cloned in Escherichia coli was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active Pro A. Replacement of the V. alginolyticus promoter region by the α-amylase promoter region from Bacillus amyloliquefaciens resulted in the simultaneous synthesis and secretion of Pro A in E. coli. The V. alginolyticus proA gene cloned on a shuttle vector did not produce active Pro A in Bacillus subtilis. Although Pro A has a typical Gram-positive signal sequence, it was not functional in B. subtilis. Replacement of the Pro A signal sequence with the α-amylase signal sequence resulted in the production of active Pro A in B. subtilis. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-2-319