1887

Abstract

A 4-hydroxy-3-methoxycinnamic acid (ferulic acid) esterase has been purified from the extracellular broth of cultures of after growth on oat spelt xylan. The purification procedure utilizes ion exchange on DEAE-BioGel A, anion exchange on Mono Q, gel filtration and hydrophobic interaction chromatography. The purified enzyme appeared as a single band on SDS-PAGE, with an apparent of 29000. Two bands, at pI 7·9 and 8·5, were observed on isoelectric focusing. With methyl ferulate as substrate, the pH and temperature optima were 5·5 and 30 °C respectively, with a of 1·86 mm and of 0·3 μmol min mg. The purified enzyme released ferulic acid from de-starched wheat bran only in the presence of xylanase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-10-2339
1991-10-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/10/mic-137-10-2339.html?itemId=/content/journal/micro/10.1099/00221287-137-10-2339&mimeType=html&fmt=ahah

References

  1. Adams G. A. 1965; Xylans. Methods of Carbohydrate Analysis V170–175 Whistler R. L. New York & London: Academic Press;
    [Google Scholar]
  2. Biely P., Mackenzie C. R., Puls J., Schneider H. 1986; Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Bio/Technology 4:731–733
    [Google Scholar]
  3. Borneman W. S., Akin D. E., VanEseltine W. P. 1986; Effect of phenolic monomers on ruminal bacteria. Applied and Environmental Microbiology 52:1331–1339
    [Google Scholar]
  4. Borneman W. S., Hartley R. D., Morrison W. H., Akin D. E., Ljungdahl L. G. 1990a; Feruloyl and pcoumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation. Applied Microbiology and Biotechnology 33:345–351
    [Google Scholar]
  5. Borneman W. S., Hartley R. D., Himmelsbach D. S., Ljungdahl L. G. 1990b; Assay for trans pcoumaroyl esterase using a specific substrate from plant cell walls. Analytical Biochemistry 190:129–133
    [Google Scholar]
  6. Chesson A., Stewart C. S., Walace R. J. 1982; Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria. Applied and Environmental Microbiology 44:597–603
    [Google Scholar]
  7. Deobald L. A., Crawford D. L. 1987; Activities of cellulase and other extracellular enzymes during lignin solubilization by Streptomyces virodosporus . Applied Microbiology and Biotechnology 26:158–163
    [Google Scholar]
  8. Donnelly P. K., Crawford D. L. 1988; Production by Streptomyces viridosporus T7A of an enzyme which cleaves aromatic acids from lignocellulose. Applied and Environmental Microbiology 54:2237–2244
    [Google Scholar]
  9. Fry S. C. 1982; Phenolic components of the primary cell wall. Biochemical Journal 203:493–504
    [Google Scholar]
  10. Greve L. C., Labavitch J. M., Hungate R. E. 1984; α lArabinofuranosidase from Ruminococcus albus 8: purification and possible role in hydrolysis of Alfalfa cell wall. Applied and Environmental Microbiology 47:1135–1140
    [Google Scholar]
  11. Hartley R. D., Jones E. C. 1977; Phenolic components and degradability of cell walls of grass and legume species. Phytochemistry 16:1531–1534
    [Google Scholar]
  12. Hartley R. D., Morrison W. H. III, Himmelsbach D. S., Borneman W. S. 1990; Cross-linking of cell wall phenolic arabinoxylans in graminaceous plants. Phytochemistry 29:3705–3709
    [Google Scholar]
  13. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich, UK: John Innes Foundation;
    [Google Scholar]
  14. Hoseney R. C., Faubion J. M. 1981; A mechanism for the oxidative gelation of wheat flour water-soluble pentosans. Cereal Chemistry 58:421–423
    [Google Scholar]
  15. Iiyama K., Lam T. B., Stone B. A. 1990; Phenolic acid bridges between polysaccharides and lignin in wheat intemodes. Phytochemistry 29:733–737
    [Google Scholar]
  16. Ishaque M., Kleupfel D. 1980; Cellulase complex of a mesophilic Streptomyces strain. Canadian Journal of Microbiology 30:183–189
    [Google Scholar]
  17. Ishii T., Hiroi T. 1990; Linkage of phenolic acids to cell-wall polysaccharides of bamboo shoot. Carbohydrate Research 206:297–310
    [Google Scholar]
  18. Johnson K. G., Fontana J. D., Mackenzie C. R. 1988a; Measurement of acetylxylan esterase in Streptomyces . Methods in Enzymology 160:551–560
    [Google Scholar]
  19. Johnson K. G., Harrison B. A., Schneider H., Mackenzie C. R., Fontana J. D. 1988b; Xylan-hydrolysing enzymes from Streptomyces spp. Enzyme and Microbial Technology 10:403–409
    [Google Scholar]
  20. Johnson K. G., Silva M. C., Mackenzie C. R., Schneider H., Fontana J. D. 1989; Microbial degradation of hemicellulosic materials. Applied Biochemistry and Biotechnology 20/21:245–258
    [Google Scholar]
  21. Jung H.-J. G., Sahlu T. 1986; Depression of cellulose digestion by esterified cinnamic acids. Journal of the Science of Food and Agriculture 37:659–665
    [Google Scholar]
  22. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  23. Lin T. S., Kolattukudy P. E. 1980; Isolation and characterization of a cuticular polyester (cutin) hydrolysing enzyme from phytopathogenic fungi. Physiological Plant Pathology 17:1–15
    [Google Scholar]
  24. Mackenzie C. R., Bilous D. 1988; Ferulic acid esterase activity from Schizophyllum commune . Applied and Environmental Microbiology 54:1170–1173
    [Google Scholar]
  25. Mackenzie C. R., Bilous D., Schneider H., Johnson K. G. 1987; Induction of cellulolytic and xylanolytic enzyme systems in Streptomyces spp. Applied and Environmental Microbiology 53:2835–2839
    [Google Scholar]
  26. McCarthy A. J. 1987; Lignocellulose-degrading actinomycetes. FEMS Microbiology Reviews 46:145–163
    [Google Scholar]
  27. McQueen D. A. R., Schottel J. L. 1987; Purification and characterization of a novel extracellular esterase from pathogenic Streptomyces scabies that is inducible by zinc. Journal of Bacteriology 169:1967–1971
    [Google Scholar]
  28. Miller G. L. 1959; Use of dinitrosalicylic reagent for determination of reducing groups. Analytical Chemistry 31:426–428
    [Google Scholar]
  29. Mueller-Harvey I., Hartley R. D., Harris P. J., Curzon E. H. 1986; Linkage of pcoumaroyl and feruloyl groups to cell-wall polysaccharides of barley straw. Carbohydrate Research 148:71–85
    [Google Scholar]
  30. Paice M. G., Jurasek M. R., Carpenter M. R., Smillie L. B. 1978; Production, characterization, and partial amino acid sequence of xylanase A from Schizophyllum commune . Applied and Environmental Microbiology 36:802–808
    [Google Scholar]
  31. Rombouts F. M., Thibault J.-F. 1986; Feruloylated pectic substances from sugar beet pulp. Carbohydrate Research 154:189–203
    [Google Scholar]
  32. Scalbert A., Monties B., Lallemand J.-Y., Guittet E., Rolando C. 1985; Ether linkage between phenolic acids and lignin fractions from wheat straw. Phytochemistry 24:1359–1362
    [Google Scholar]
  33. Smith M. M., Hartley R. D. 1983; Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in graminaceous plants. Carbohydrate Research 118:65–80
    [Google Scholar]
  34. Watanabe T., Ohnishi J., Yamasaki Y., Kaizu S., Koshijima T. 1989; Binding-site analysis of the ether linkages between lignin and hemicelluloses in lignin–carbohydrate complexes by DDQ-oxidation. Agricultural and Biological Chemistry 53:2233–2252
    [Google Scholar]
  35. Wilkinson G. N. 1961; Statistical estimations in enzyme kinetics. Biochemical Journal 80:324–332
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-10-2339
Loading
/content/journal/micro/10.1099/00221287-137-10-2339
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error