RT Journal Article SR Electronic(1) A1 Faulds, Craig B. A1 Williamson, GaryYR 1991 T1 The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes JF Microbiology, VO 137 IS 10 SP 2339 OP 2345 DO https://doi.org/10.1099/00221287-137-10-2339 PB Microbiology Society, SN 1465-2080, AB A 4-hydroxy-3-methoxycinnamic acid (ferulic acid) esterase has been purified from the extracellular broth of cultures of Streptomyces olivochromogenes after growth on oat spelt xylan. The purification procedure utilizes ion exchange on DEAE-BioGel A, anion exchange on Mono Q, gel filtration and hydrophobic interaction chromatography. The purified enzyme appeared as a single band on SDS-PAGE, with an apparent M r of 29000. Two bands, at pI 7·9 and 8·5, were observed on isoelectric focusing. With methyl ferulate as substrate, the pH and temperature optima were 5·5 and 30 °C respectively, with a K m of 1·86 mm and V max of 0·3 μmol min–1 mg–1. The purified enzyme released ferulic acid from de-starched wheat bran only in the presence of xylanase., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-10-2339