1887

Abstract

Summary: Glycollate inhibited growth of in media containing either pyruvate or lactate as carbon sources. Glycollamide, but not glyoxylate, showed similar effects. Spontaneous mutants (L/G strains) were isolated that were able to grow on lactate medium in the presence of glycollate: their growth in pyruvate medium was still inhibited by glycollate. Synthesis of membrane-bound NAD-independent (−)- and (+)-lactate dehydrogenases (iLDHs) was inducible by - or -lactate in the parent strain but was constitutive in the L/G strains. Glycollate inhibited induction of the synthesis of iLDHs in the parent strain growing in succinate medium but had no effect under the same conditions on strain L/G1. Glycollate was a competitive inhibitor of (+)-iLDH ( = 11 mM). No differences were found in the kinetic properties of (+)-iLDH in cell-free extracts from strain L/G1 and the parent organism. Glycollate appears to inhibit growth on lactate medium predominantly through prevention of lactate induction of iLDH synthesis.

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/content/journal/micro/10.1099/00221287-133-6-1521
1987-06-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-6-1521
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