@article{mbs:/content/journal/micro/10.1099/00221287-133-6-1521, author = "Brown, P. R. and Tata, Renée", title = "Glycollate Inhibition of Growth of Pseudomonas aeruginosa on Lactate Medium", journal= "Microbiology", year = "1987", volume = "133", number = "6", pages = "1521-1526", doi = "https://doi.org/10.1099/00221287-133-6-1521", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-6-1521", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Glycollate inhibited growth of Pseudomonas aeruginosa in media containing either pyruvate or lactate as carbon sources. Glycollamide, but not glyoxylate, showed similar effects. Spontaneous mutants (L/G strains) were isolated that were able to grow on lactate medium in the presence of glycollate: their growth in pyruvate medium was still inhibited by glycollate. Synthesis of membrane-bound NAD+-independent D(−)- and L(+)-lactate dehydrogenases (iLDHs) was inducible by D- or L-lactate in the parent strain but was constitutive in the L/G strains. Glycollate inhibited induction of the synthesis of iLDHs in the parent strain growing in succinate medium but had no effect under the same conditions on strain L/G1. Glycollate was a competitive inhibitor of L(+)-iLDH (K i = 11 mM). No differences were found in the kinetic properties of L(+)-iLDH in cell-free extracts from strain L/G1 and the parent organism. Glycollate appears to inhibit growth on lactate medium predominantly through prevention of lactate induction of iLDH synthesis.", }