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Staphylococcus aureus (Wood 46) was grown aerobically and anaerobically in supplemented 3% (w/v) Tryptone Soya Broth medium for 24 h at 37°C. Although the bacterial density achieved was 9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1·4 times higher than in the anaerobic culture. However, the SDS-PAGE patterns of extracellular proteins were quite different : the aerobic products occurred almost exclusively in the mol. wt range 15–30000 compared with 30–60000 for those produced anaerobically. The only major component common to both preparations was α-toxin which accounted for 2·4 times more of the total exoprotein under aerobic than under anaerobic conditions.
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