-
Volume 131,
Issue 2,
1985
Volume 131, Issue 2, 1985
- Biochemistry
-
-
-
Inhibition of Glucosamine Synthase by Bacilysin and Anticapsin
More Lessl-Glutamine d-fructose-6-phosphate amidotransferase (‘glucosamine synthase’, EC 5.3.1.19) from Escherichia coli MRE 600 was purified at least 75-fold. It catalysed the formation of 21·1 μmol glucosamine 6-phosphate (mg protein)–1 in 30 min at 37 °C. Its molecular weight, estimated by gel filtration, was about 90000 and it was inhibited by thiol group reagents. Anticapsin, the C-terminal amino acid of the dipeptide antibiotic bacilysin, and to a lesser extent bacilysin itself, inhibited glucosamine synthase activity. Kinetic studies indicated that the inhibition was non-competitive with respect to fructose 6-phosphate as substrate but partly competitive with respect to l-glutamine. Incubation of the enzyme with anticapsin brought about a time-dependent and irreversible inhibition. It is suggested that anticapsin behaves as a glutamine analogue and that a reaction of its epoxide group with a thiol group of glucosamine synthase results in its linkage to the enzyme by a covalent bond.
-
-
-
-
Purification and Characterization of Glucosyltransferases from Streptococcus mutans 6715
More LessSummary: A water-soluble glucan-synthesizing glucosyltransferase (GTase-S) and a water-insoluble glucan-synthesizing glucosyltransferase (GTase-I) were purified from culture supernatant of Streptococcus mutans 6715 (serotype g) by ammonium sulphate precipitation, chromatofocusing on a Polybuffer exchanger PBE 94 column, and subsequent phenyl-Sepharose CL-4B or hydroxyapatite column chromatography. The GTase-S and GTase-I activities were purified 4019- and 4714-fold, respectively, and the molecular weights were calculated to be 160000 and 165000, respectively. GTase-S had a pH optimum of 5·0, a K m of 8·8 mm for sucrose in the presence of 20μm-dextran T10, and an isoelectric point of pH 4·3. GTase-I had two pH optima of 5·0 and 7·0, K m values of 9·4 mm (at pH 5·0) and 7·0 mm (at pH 7·0), and an isoelectric point of pH 4·9. Methylation analysis indicated that the water-soluble glucan produced by GTase-S was a highly branched 1,6-α-linked d-glucan with 1,3-linked glucose residues, and that the water-insoluble glucan synthesized by GTase-I was composed of 1,3-α-linked glucose units.
-
-
-
Occurrence of Extracellular (1→2)-β-d-Glucans and (1→2)-β-d-Gluco-oligosaccharides in Acetobacter
More LessSummary: Of 11 strains of Acetobacter tested, three strains of A. xylinum (IFO 3288, IFO 13693 and IFO 13772), two strains of A. aceti (IFO 3281 and IFO 3283), one strain of A. pasteurianus (IFO 3223) and one strain of A. rancens (IFO 3297) were found to produce 2–25 mg per 100 ml culture filtrate of a mixture of (1→2)-β-d-glucans and (1→2)-β-d-gluco-oligosaccharides as low-molecular-weight products. The glucans and oligosaccharides were linear without branching structures and their degrees of polymerization (DPs) ranged from 6 to about 42. The compounds with lower DPs did not seem to be produced by hydrolysis of those with higher DPs. A. xylinum IFO 3288 and A. xylinum IFO 13693 also produced extracellular acidic polysaccharides containing l-rhamnose, d-glucose, d-mannose and uronic acid.
-
-
-
Glucose-6-phosphate Dehydrogenase from Agaricus bisporus: Purification and Properties
More LessAgaricus bisporus glucose-6-phosphate dehydrogenase (EC 1.1.1.49) was purified to homogeneity. The subunit molecular weight was 55300 and the molecular weight of the native protein was 138000 by gel filtration and 131 800 by rate zonal ultracentrifugation on sucrose gradients. There was some evidence for a small population of higher molecular weight oligomers during rate zonal centrifugation. A sedimentation coefficient of 8·5S was determined by analytical ultracentrifugation (suggesting the presence of the tetramer). Three bands of enzyme activity were separated on polyacrylamide gels. Digests of the proteins in the three bands gave peptide patterns with no detectable difference after SDS electrophoresis. Linear double reciprocal plots were obtained with glucose 6-phosphate as a variable substrate. The apparent Km for glucose 6-phosphate was 214 μm and the V max was 520 μmol (mg protein)–1 min–1. A sigmoidal saturation curve and a non-linear double reciprocal plot were observed for NADP, giving a Hill constant of 1·5 and [S]0·5 for NADP of 89 μm. The enzyme was inhibited by NADPH, with 50% inhibition at a NADP: NADPH ratio of 0·4 when total NADP(H) concentration was 0·2 mm. ATP, ADP, AMP and cAMP had no effect on enzyme activity. Palmitoyl CoA inhibited the initial velocity of the reaction by 95% at a concentration of 1·5 μm when incubated with enzyme prior to NADP addition.
-
-
-
Sugar, Sugar Phosphate and NADP(H) Levels in Agaricus bisporus Fruit Bodies
More LessLevels of glucose 6-phosphate, fructose 6-phosphate, 6-phosphogluconate, fructose 1,6-bisphosphate, trehalose, glucose, fructose, mannitol, NADP and NADPH were determined in Agaricus bisporus fruit body tissues. Of the phosphorylated sugars, glucose 6-phosphate was present in the largest quantity, at approximately 1·5 μmol (g fresh wt)–1. Fructose was detected in larger quantities than in previous investigations. NADP and NADPH levels were found to be in the range 45–200 nmol (g fresh wt)–1. with NADP/NADPH ratios between 0·58 and 1·66. The anabolic reduction charge showed a negative relationship with mannitol level. Mass action ratios calculated for glucose-6-phosphate dehydrogenase were several orders of magnitude below the reported k eq for the reaction. The mass action ratio for glucose-6-phosphate dehydrogenase showed a significant correlation with trehalose content over the period of sampling. The results are discussed with reference to possible controls of the pentose phosphate pathway and mannitol synthesis.
-
-
-
Subcellular Fractionation of Candida boidinii after Growth on Glucose or Methanol
More LessExtracts of glucose-grown and methanol-grown Candida boidinii were fractionated on sucrose gradients. Mitochondria and microbodies from methanol-grown cells were more fragile than those from glucose-grown cells. Formaldehyde and formate dehydrogenases and antimycin A-insensitive NADPH: cytochrome c oxidoreductase were non-sedimentable enzymes. The mitochondrial zone defined by cytochrome c oxidase (ρ = 1·19 to 1·20 g ml–1) contained sedimentable activities of malate dehydrogenase and citrate synthase. The vacuolar enzyme acid phosphatase was also recovered from this region. The microbody enzyme catalase sedimented to a lower density (ρ = 1·17 g ml–1) than mitochondria after growth on glucose but, together with methanol oxidase activity, to higher densities (ρ = 1·23 and 1·27 g ml–1) after growth on methanol. Activity of the methanol assimilation enzyme dihydroxyacetone synthase was partially sedimentable and was recovered in the fraction containing mitochondria and membraneous vesicles. Inosine diphosphatase was probably multilocational in the cells.
-
-
-
Composition of Membranes from Whole Cells and Minicells of Bacillus subtilis
More LessThe composition of membranes from whole cells and minicells of Bacillus subtilis strain BD474 was examined. Two-dimensional electrophoresis showed that the content of several individual polypeptides differed in the two membrane types. Whole cell membranes appeared to contain more penicillin binding protein (PBP) 2a, glycerol-3-phosphate dehydrogenase, succinate dehydrogenase and NADH dehydrogenase than minicell membranes, but less PBP2b, lactate dehydrogenase and malate dehydrogenase. Glycerol-3-phosphate dehydrogenase and succinate dehydrogenase were unstable, but the other enzymes and PBPs were stable after inhibition of protein synthesis by chloramphenicol. Minicells synthesized peptidoglycan because they incorporated 14C-labelled acetylglucosamine into acid precipitable material. However, the rate of incorporation in minicells was only 5% of the value observed in whole cells. Whole cell and minicell membranes contained the same polar lipids and fatty acids, but the whole cell membranes were enriched in phosphatidylglycerol, diglycosyldiacylglycerol, iso-C15 and anteiso-C15 fatty acids, but depleted of phosphatidylethanolamine, iso-C14, iso-C16, n-C16 and anteiso-C17 fatty acids.
-
- Development And Structure
-
-
-
Alterations in Trehalase Solubility during Development in the Cellular Slime Mould Dictyostelium discoideum
More LessPrevious studies have indicated that during development in the slime mould Dictyostelium discoideum, compartmentation of the isoenzymes of trehalase (α,α′-trehalose 1-d-glucohydrolase, (EC 3.2.1.28) occurs between the extracellular and intracellular environments. The compartmentation of trehalase between soluble and particulate cell fractions was examined in this work. The trehalase present in crude homogenates prepared during the first 12 h of development was completely soluble. Starting at about the pseudoplasmodial stage (i.e. the 14th hour of development), trehalase activity became associated with insoluble cellular material and this increased to a maximal value in homogenates from mature sorocarps, where 50% of the activity was insoluble. Spore cells accounted for only 2 to 3% of the trehalase associated with mature sorocarps, with the remaining 97% being localized in stalk cell material. Although trehalase recovered from spores was completely soluble, more than half of that from the stalk was recovered in the buffer-insoluble pellet fraction.
-
-
-
-
A Correlation between Mode of Growth and Regional Ultrastructure of the Plasma Membrane of Schizosaccharomyces pombe as Revealed by Freeze-fracturing before and after Filipin Treatment
More LessThe ultrastructure of the plasma membrane of Schizosaccharomyces pombe was studied by freeze-fracture using invaginations of the plasma membrane as natural markers and filipin-induced deformations as artificial markers. In accord with the mode of growth of this organism, ultra-structural aspects of the plasma membrane were related to the following ring zones: the growing pole, adjacent regions, proximal regions, the new cell pole, and the middle in dividing cells. The growing pole and adjacent regions had no or only a few invaginations. Filipin induced numerous deformations in these regions. By contrast, the proximal regions of the plasma membrane had several invaginations and resisted filipin-induced deformation. Concomitantly with commitment to cytokinesis, both the invaginations and the resistance to filipin-induced deformation disappeared in the middle. The results presented here strongly suggest the existence of two states of the plasma membrane of S. pombe, a fact which correlates well with the mode of growth of this organism.
-
- Genetics And Molecular Biology
-
-
-
Characterization of a Salmonella typhimurium Mutant Defective in Phosphoribosylpyrophosphate Synthetase
More LessThis study describes the isolation and characterization of a mutant (strain GP122) of Salmonella typhimurium with a partial deficiency of phosphoribosylpyrophosphate (PRPP) synthetase activity. This strain was isolated in a purE deoD gpt purine auxotroph by a procedure designed to select guanosine-utilizing mutants. Strain GP122 had roughly 15% of the PRPP synthetase activity and 25% of the PRPP pool of its parent strain. The mutant exhibited many of the predicted consequences of a decreased PRPP pool and a defective PRPP synthetase enzyme, including: (1) poor growth on purine bases; (2) decreased accumulation of 5-aminoimidazole ribonucleotide (the substrate of the blocked purE reaction) under conditions of purine starvation; (3) excretion of anthranilic acid when grown in medium lacking tryptophan; (4) increased resistance to inhibition by 5-fluorouracil ; (5) derepressed levels of aspartate transcarbamylase and orotate phosphoribosyltransferase, enzymes involved in the pyrimidine de novo biosynthetic pathway;(6) growthstimulation by PRPP-sparing compounds(e.g.guanosine,histidine); (7)poor growth in low phosphate medium; and (8) increased heat lability of the defective enzyme. This mutant strain also had increased levels of guanosine 5′-monophosphate reductase. This genetic lesion, designated prs, was mapped by conjugation and phage P22-mediated transduction at 35 units on the Salmonella linkage map.
-
-
-
-
Expression of a Gene for Glucan-binding Protein from Streptococcus mutans in Escherichia coli
More LessSummary: The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage λ vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.
-
-
-
Introduction of the Hairy Root Plasmid into Rhizobium meliloti Results in Increased Nodulation on its Host
More LessWe have introduced the hairy root plasmid (pRi) from Agrobacterium rhizogenes into Rhizobium meliloti. Transfer was accomplished by the use of a Tn5 mutant of the plasmid which had improved transfer abilities. The R. meliloti (pRi) transconjugants did not induce root proliferation in any of the plant species tested, including alfalfa (Medicago sativa). The transconjugants nodulated alfalfa and the nodules appeared to be identical, morphologically and cytologically, to those produced by the parent R. meliloti strain. In growth chamber tests, the R. meliloti (pRi) transconjugants induced significantly more nodules per plant on alfalfa than either the R. meliloti parent or the wild-type strain of R. meliloti. The hairy root plasmid was stably maintained in R. meliloti after being passed serially through two plantings of alfalfa.
-
-
-
Association of the Encapsulation of Bacillus anthracis with a 60 Megadalton Plasmid
More LessVirulent typical strains (Shikan, Morioka, Shizuoka) and Pasteur vaccine strains (no. 1, no. 2-H, no. 2-17JB) of Bacillus anthracis harboured two plasmid species with molecular masses of 110 MDal and 60 MDal. All of the 110 MDal plasmids isolated from the various strains showed indistinguishable patterns of digestion with restriction endonucleases. All the 60 MDal plasmids were also indistinguishable. Strain Davis, which is encapsulated but is asporogenous and avirulent, harboured only the 60 MDal plasmid while three non-encapsulated vaccine strains (34F2, Smith, Mukteswer) harboured only the 110 MDal plasmid. Four non-encapsulated variant strains obtained from the encapsulated strains Shikan, Pasteur no. 1, Pasteur no. 2-17JB and Davis had lost the 60 MDal plasmid, suggesting that encapsulation of B. anthracis may be associated with the 60 MDal plasmid.
-
-
-
Escherichia coli K12 Strains for Use in the Identification and Characterization of Colicins
More LessA collection of strains derived from Escherichia coli K12 W3110 and harbouring various colicin or microcin plasmids (18 and 2 representatives, respectively), or carrying well-characterized mutations conferring reduced colicin/microcin sensitivity is described. The strains can be used in typing schemes based on the identification of colicins, in the detection of new types of colicins/microcins, and in the further characterization of previously identified colicins/microcins and their plasmids.
-
- Pathogenicity And Medical Microbiology
-
-
-
ELISA for the Detection of Specific IgM and IgG in Human Leptospirosis
More LessELISA was used to detect specific IgM and IgG in sera from humans with current or past leptospirosis. A serological pattern of a high IgM titre (⩾ 1280), or moderately increased IgM (160–640) in conjunction with a low IgG titre (⩽ 20), with serovar copenhageni antigen was characteristic for approximately two-thirds of the sera from serovar icterohaemorrhagiae patients obtained in the first two months of the disease. The antigen was the supernatant of a heated and centrifuged culture of leptospires. Antigens were prepared from serovars copenhageni, grippotyphosa, hardjo and patoc. Sera from patients with icterohaemorrhagiae, grippotyphosa and hardjo infections showed cross-reactivity when different antigens were used. In past infections the IgG titres were clearly higher with the homologous antigen. ELISA for IgM and IgG allows the rapid diagnosis of acute leptospirosis.
-
-
-
-
Purification, Characterization and Immunological Properties of the Serotype-specific Capsular Polysaccharide of Pasteurella haemolytica (Serotype T4) Organisms
More LessThe serotype-specific capsular polysaccharide from two strains of Pasteurella haemolytica serotype T4 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer, a teichoic acid, has the backbone structure →(2-glycerol- l)→(phosphate)→(6-α-d-galactose-l)→ and is partially O-acetylated on the C2 and C3 galactose residues. Chemical removal of O-acetyl groups from the polysaccharide destroyed both its ability to precipitate with antiserum raised against killed whole serotype T4 organisms and its ability to adhere to sheep erythrocytes in passive haemagglutination experiments. Attempts to elicit antisera using the purified polymer were unsuccessful but a partially purified material was immunogenic.
-
-
-
The Toxic Role of Alpha-haemolysin in the Pathogenesis of Experimental Escherichia coli Infection in Mice
More LessFiltrates from strains of Escherichia coli possessing plasmid-cloned haemolysin (Hly) genes and from strains possessing ‘wild’ Hly plasmids were lethal for mice on intravenous inoculation; similar doses of preparations from derivatives of these strains in which the Hly genes had been rendered non-functional or which did not possess the ‘wild’ plasmids were not. Live cultures of both kinds of Hly+ strain usually had a lower lethal dose for mice on intraperitoneal inoculation than the corresponding Hly– forms. Mice that had been inoculated with Hly+ forms had shorter survival times and lower numbers of organisms in peritoneal washings, lungs and blood at point of death than mice that had been inoculated with the corresponding Hly–forms; this was also so for mice pre-treated with FeSO4, a procedure which rendered mice equally susceptible to the lethal effects of the Hly+ and Hly– forms of a strain. In FeSO4-treated mice the numbers of organisms in the tissues of those dying from infection with Hly+ organisms were no higher than they were at the same time after inoculation in others given the corresponding Hly− forms; before mice of the latter category died the numbers of organisms in their tissues increased greatly. The clinical and pathological signs exhibited by mice inoculated with Hly+ organisms, but not with Hly– organisms, resembled those exhibited by mice inoculated with bacteria-free haemolysin preparations. These results suggest that haemolysin played a significant role in the pathogenesis of the disease produced by the Hly+ organisms by having a direct toxic action on the host.
-
- Physiology And Growth
-
-
-
Influence of Alkali Metal Cations on the Germination of Spores of Wild-type and gerD Mutants of Bacillus subtilis
More LessSpores of gerD mutants of Bacillus subtilis prepared on a rich medium were defective in their germination responses to l-alanine, to a mixture of l-asparagine, d-glucose, d-fructose and KC1 (AGFK), and to Penassay broth. They showed an increased response and sensitivity to lalanine when monovalent cations were also present. When prepared by a resuspension method, the spores had an improved germination response to l-alanine, even in the absence of these cations, but showed no such improvement in response to AGFK. No gross differences were found between wild-type and gerD spores in heat or chemical resistance, dipicolinic acid level or morphology. It is proposed that an alternative pathway of germination involving monovalent cations exists in both wild-type and gerD spores.
-
-
-
-
The Effect of Nickel on Hydrogen Metabolism and Nitrogen Fixation in the Cyanobacterium Anabaena cylindrica
More LessA comparative study was made of the growth and nitrogen fixation of nickel-depleted and nickel-supplemented cultures of the cyanobacterium Anabaena cylindrica. Four sets of growth conditions were used, involving both dark/light and continuous light regimes, anaerobic and aerobic conditions, light limitation and supplementation of the gas phase with hydrogen. In each case nickel-containing cells had an active hydrogen uptake capacity whereas nickel-depleted cells did not. These differences in hydrogenase activities did not correlate with differences in acetylene reduction and growth rates, or fixed nitrogen, phycocyanin or chlorophyll contents. It is concluded that under the growth conditions used the capacity of cells to consume hydrogen gas confers no advantage to the organisms in terms of their growth rates and nitrogen fixation.
-
-
-
The ftsA Gene Product: a Possible Connection between DNA Replication and Septation in Escherichia coli
More LessThe study of Escherichia coli strain D-2, which harbours the ftsA2(ts) allele, has shown that temperature-induced filaments of this strain can divide, at 30 °C, in the absence of DNA replication and translation. Strain D-2 is thermosensitive during a period coincident with that in which the termination protein should be synthesized and exert its action. The ftsA gene product, which participates in the structure of the septum, needs for its synthesis a short period of DNA replication. The FtsA protein could be involved in a mechanism that coordinates chromosome replication and cell division by a pathway different from and independent of the SOS-induced response.
-
Volumes and issues
-
Volume 171 (2025)
-
Volume 170 (2024)
-
Volume 169 (2023)
-
Volume 168 (2022)
-
Volume 167 (2021)
-
Volume 166 (2020)
-
Volume 165 (2019)
-
Volume 164 (2018)
-
Volume 163 (2017)
-
Volume 162 (2016)
-
Volume 161 (2015)
-
Volume 160 (2014)
-
Volume 159 (2013)
-
Volume 158 (2012)
-
Volume 157 (2011)
-
Volume 156 (2010)
-
Volume 155 (2009)
-
Volume 154 (2008)
-
Volume 153 (2007)
-
Volume 152 (2006)
-
Volume 151 (2005)
-
Volume 150 (2004)
-
Volume 149 (2003)
-
Volume 148 (2002)
-
Volume 147 (2001)
-
Volume 146 (2000)
-
Volume 145 (1999)
-
Volume 144 (1998)
-
Volume 143 (1997)
-
Volume 142 (1996)
-
Volume 141 (1995)
-
Volume 140 (1994)
-
Volume 139 (1993)
-
Volume 138 (1992)
-
Volume 137 (1991)
-
Volume 136 (1990)
-
Volume 135 (1989)
-
Volume 134 (1988)
-
Volume 133 (1987)
-
Volume 132 (1986)
-
Volume 131 (1985)
-
Volume 130 (1984)
-
Volume 129 (1983)
-
Volume 128 (1982)
-
Volume 127 (1981)
-
Volume 126 (1981)
-
Volume 125 (1981)
-
Volume 124 (1981)
-
Volume 123 (1981)
-
Volume 122 (1981)
-
Volume 121 (1980)
-
Volume 120 (1980)
-
Volume 119 (1980)
-
Volume 118 (1980)
-
Volume 117 (1980)
-
Volume 116 (1980)
-
Volume 115 (1979)
-
Volume 114 (1979)
-
Volume 113 (1979)
-
Volume 112 (1979)
-
Volume 111 (1979)
-
Volume 110 (1979)
-
Volume 109 (1978)
-
Volume 108 (1978)
-
Volume 107 (1978)
-
Volume 106 (1978)
-
Volume 105 (1978)
-
Volume 104 (1978)
-
Volume 103 (1977)
-
Volume 102 (1977)
-
Volume 101 (1977)
-
Volume 100 (1977)
-
Volume 99 (1977)
-
Volume 98 (1977)
-
Volume 97 (1976)
-
Volume 96 (1976)
-
Volume 95 (1976)
-
Volume 94 (1976)
-
Volume 93 (1976)
-
Volume 92 (1976)
-
Volume 91 (1975)
-
Volume 90 (1975)
-
Volume 89 (1975)
-
Volume 88 (1975)
-
Volume 87 (1975)
-
Volume 86 (1975)
-
Volume 85 (1974)
-
Volume 84 (1974)
-
Volume 83 (1974)
-
Volume 82 (1974)
-
Volume 81 (1974)
-
Volume 80 (1974)
-
Volume 79 (1973)
-
Volume 78 (1973)
-
Volume 77 (1973)
-
Volume 76 (1973)
-
Volume 75 (1973)
-
Volume 74 (1973)
-
Volume 73 (1972)
-
Volume 72 (1972)
-
Volume 71 (1972)
-
Volume 70 (1972)
-
Volume 69 (1971)
-
Volume 68 (1971)
-
Volume 67 (1971)
-
Volume 66 (1971)
-
Volume 65 (1971)
-
Volume 64 (1970)
-
Volume 63 (1970)
-
Volume 62 (1970)
-
Volume 61 (1970)
-
Volume 60 (1970)
-
Volume 59 (1969)
-
Volume 58 (1969)
-
Volume 57 (1969)
-
Volume 56 (1969)
-
Volume 55 (1969)
-
Volume 54 (1968)
-
Volume 53 (1968)
-
Volume 52 (1968)
-
Volume 51 (1968)
-
Volume 50 (1968)
-
Volume 49 (1967)
-
Volume 48 (1967)
-
Volume 47 (1967)
-
Volume 46 (1967)
-
Volume 45 (1966)
-
Volume 44 (1966)
-
Volume 43 (1966)
-
Volume 42 (1966)
-
Volume 41 (1965)
-
Volume 40 (1965)
-
Volume 39 (1965)
-
Volume 38 (1965)
-
Volume 37 (1964)
-
Volume 36 (1964)
-
Volume 35 (1964)
-
Volume 34 (1964)
-
Volume 33 (1963)
-
Volume 32 (1963)
-
Volume 31 (1963)
-
Volume 30 (1963)
-
Volume 29 (1962)
-
Volume 28 (1962)
-
Volume 27 (1962)
-
Volume 26 (1961)
-
Volume 25 (1961)
-
Volume 24 (1961)
-
Volume 23 (1960)
-
Volume 22 (1960)
-
Volume 21 (1959)
-
Volume 20 (1959)
-
Volume 19 (1958)
-
Volume 18 (1958)
-
Volume 17 (1957)
-
Volume 16 (1957)
-
Volume 15 (1956)
-
Volume 14 (1956)
-
Volume 13 (1955)
-
Volume 12 (1955)
-
Volume 11 (1954)
-
Volume 10 (1954)
-
Volume 9 (1953)
-
Volume 8 (1953)
-
Volume 7 (1952)
-
Volume 6 (1952)
-
Volume 5 (1951)
-
Volume 4 (1950)
-
Volume 3 (1949)
-
Volume 2 (1948)
-
Volume 1 (1947)
Most Read This Month
