1887

Abstract

Summary: A mutant of lacking aconitase did not grow on minimal medium (MM) and had five-to tenfold less NADP-depcndent glutamate dehydrogenase (GDH) activity than the wild-type, although its glutamine synthetase (GS) activity was still inducible. When this mutant was incubated with glutamate as the sole nitrogen source, the 2-oxoglutarate content rose, and the NADP-dependent GDH activity increased. Furthermore, carbon-limited cultures showed a direct relation between NADP-dependent GDH activity and the intracellular 2-oxoglutarate content. We propose that the low NADP-dependent GDH activity found in the mutant was due to the lack of 2-oxoglutarate or some other intermediate of the tricarboxylic acid cycle.

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1985-10-01
2021-05-13
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References

  1. Anfinsen C. B. 1955; Aconitase from pig heart muscle. Methods in Enzymology 1:695–698
    [Google Scholar]
  2. Doherty D. 1970; L-Glutamate dehydrogenases (yeast). Methods in Enzymology 17:850–856
    [Google Scholar]
  3. Dubois E., Grenson M., Wiame J.M. 1974; The participation of the anabolic glutamate dehydrogenase in the nitrogen catabolite repression of arginase in Saccharomyces cerevisiae. European Journal of Biochemistrr 48:603–616
    [Google Scholar]
  4. Ferguson A. R., Sims A. P. 1974; The regulation of glutamine metabolism in Candida utilis: the role of glutamine in the control of glutamine synthetase. Journal of General Microbiology 80:159–171
    [Google Scholar]
  5. Fink G. R. 1970; The biochemical genetics of yeast. Methods in Enzymology 17:59–78
    [Google Scholar]
  6. GonzÁlez A., Tenorio M., Vaca G., Mora J. 1983; Neurospora crassa mutant impaired in ghitamine regulation. Journal of Bacteriology 155:1–7
    [Google Scholar]
  7. Hemmings B. A. 1978; Evidence for the degradation of nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenase of Candida utilis during rapid enzyme inactivation. Journal of Bacteriology 133:867–877
    [Google Scholar]
  8. HernÁndez G., SÁnchez-Pescador R., Palacios R., Mora J. 1983; Nitrogen source regulates glutamate dehydrogenase NADP synthesis in Neurospora crassa. Journal of Bacteriology 154:524–528
    [Google Scholar]
  9. Holzer H., Schneider S. 1957; Anreicherung und Trennung einer DPN-spezifischen und einer TPN-spezifischen Glutaminsaure Dehydrogenase aus Hefe. Biochemische Zeitschriji 329:361–367
    [Google Scholar]
  10. Kang L., Keeler M., Dunlop P. C., Roon R. J. 1982; Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae. Journal of Bacteriology 151:29–35
    [Google Scholar]
  11. Kapoor M., Grover A. K. 1970; Catabolite controlled regulation of glutamate dehydrogenase of Neurospora crassa. Canadian Journal of Microbiology 16:33–40
    [Google Scholar]
  12. Legrain C., Vissers S., Dubois E., Legrain M., Wiame J. M. 1982; Regulation of glutamine synthetase from Saccharomyces cerevisiae by repression, inactivation and proteolysis. European Journal of Biochemistry 123:611–616
    [Google Scholar]
  13. Mazon M. J. 1978; Effect of glucose starvation on the nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenase of yeast. Journal of Bacteriology 133:780–785
    [Google Scholar]
  14. Mazon J., Hemmings B. A. 1979; Regulation of Saccharvmyces cerevisiae nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase by proteolysis during carbon starvation. Journal of Bacteriology 139:686–689
    [Google Scholar]
  15. Messenguy F., Colin D., Tenh Ave J. P. 1980; Regulation of compartmentation of amino acid pools in Saccharomyces cerevisiae and its effects on metabolic control. European Journal of Biochemistry 108:439–445
    [Google Scholar]
  16. Ogur M., Coker L., Ogur S. 1964; Glutamate auxotrophs in Saccharomyces. I. The biochemical lesion in the gltl mutants. Biochemical and Biophysical Research Communications 141:193–197
    [Google Scholar]
  17. Pateman J. A., Kinghorn J. R. 1975; Nitrogen metabolism. In Filamentous Fungi vol. 2 pp. 159–237 Edited by Smith J. E., Berry D. London: Edward Arnold;
    [Google Scholar]
  18. Polaina J., Cokde J. 1981; Use of the polyene antibiotic N-glycosyl-polifungin in counterselecting yeast mutants. Mutation Research 91:111–114
    [Google Scholar]
  19. Roon R. J., Even H. L. 1973; Regulation of the nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenases of Saccharomyces cerevisiae. Journal of Bacteriology 116:367–372
    [Google Scholar]
  20. Sanwal B. D., Lata M. 1961; The occurrence of two different glutamic acid dehydrogenases in Neurospora crassa. Canadian Journal of Microbiology 71:319–328
    [Google Scholar]
  21. Senior P. J. 1975; Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with continuous-culture technique. Journal of Bacteriology 123:407–418
    [Google Scholar]
  22. Tachiki T., Toboro K., Horino I., Tochiburo T. 1981; Assimilation of ammonia by Gluconobacter suhoxydans through glutamine synthetase/glutamate synthetase pathway. Agricultural and Biological Chemistry 45:1715–1717
    [Google Scholar]
  23. Tempest D. W., , Meers J. L., Brown C. M. 1970; Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochemical Journal 117:405–407
    [Google Scholar]
  24. Vaca G., Mora J. 1977; Nitrogen regulation of arginase in Neurospora crassa. Journal ofBacteriology 131:719–725
    [Google Scholar]
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