1887

Abstract

Summary: A mutant of lacking aconitase did not grow on minimal medium (MM) and had five- to tenfold less NADP-dependent glutamate dehydrogenase (GDH) activity than the wild-type, although its glutamine synthetase (GS) activity was still inducible. When this mutant was incubated with glutamate as the sole nitrogen source, the 2-oxoglutarate content rose, and the NADP-dependent GDH activity increased. Furthermore, carbon-limited cultures showed a direct relation between NADP-dependent GDH activity and the intracellular 2-oxoglutarate content. We propose that the low NADP-dependent GDH activity found in the mutant was due to the lack of 2-oxoglutarate or some other intermediate of the tricarboxylic acid cycle.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-10-2565
1985-10-01
2019-11-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-10-2565
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error