%0 Journal Article %A GonzÁlez, Alicia %A RodrÍguez, Laura %A Olivera, Hiram %A SoberÓn, Mario %T NADP+-dependent Glutamate Dehydrogenase Activity is Impaired in Mutants of Saccharomyces cerevisiae That Lack Aconitase %D 1985 %J Microbiology, %V 131 %N 10 %P 2565-2571 %@ 1465-2080 %R https://doi.org/10.1099/00221287-131-10-2565 %I Microbiology Society, %X Summary: A mutant of Saccharomyces cerevisiae lacking aconitase did not grow on minimal medium (MM) and had five-to tenfold less NADP+-depcndent glutamate dehydrogenase (GDH) activity than the wild-type, although its glutamine synthetase (GS) activity was still inducible. When this mutant was incubated with glutamate as the sole nitrogen source, the 2-oxoglutarate content rose, and the NADP+-dependent GDH activity increased. Furthermore, carbon-limited cultures showed a direct relation between NADP+-dependent GDH activity and the intracellular 2-oxoglutarate content. We propose that the low NADP+-dependent GDH activity found in the mutant was due to the lack of 2-oxoglutarate or some other intermediate of the tricarboxylic acid cycle. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-10-2565