1887

Abstract

A specialized transducing phage for the gene, specifying the sorbitol-specific Enzyme II of the phosphoenolpyruvate: sugar phosphotransferase system, was constructed and its DNA was analysed by restriction endonuclease digestion. Phage construction involved four steps: (1) integration of into the gene; (2) selection of phage carrying (a) the left and (b) the right end of the gene by means independent of the function of the new DNA acquired; (3) reconstitution of the gene in a dilysogen of these two phage; and (4) the excision, using the heteroimmune lambdoid phage 21, of a plaque-forming phage from the dilysogenic chromosome. Comparison of the DNA restriction digests of the transducing phage with those of its parents and of wild-type revealed fragments consisting partly of and partly of DNA. The junction points in the intermediate phage define a site that must lie within the reconstituted gene of the final phage. This technique should be of general application in relating genes, cloned by our method, to DNA sequences.

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1982-03-01
2021-08-03
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References

  1. Amaral D., Kornberg H. L. 1975; Regulation of fructose uptake by glucose in Escherichia coli. Journal of General Microbiology 90:157–168
    [Google Scholar]
  2. Beckwith J. R., Signer E. R., Epstein W. 1966; Transposition of the lac region of E. coli. Cold Spring Harbor Symposia on Quantitative Biology 31:393–401
    [Google Scholar]
  3. Buxton R. S., Hammer-Jespersen K., Hansen T. D. 1978; Insertion of bacteriophage lambda into the deo operon of Escherichia coli K12 and isolation of plaque forming λdeo+ transducing bacteriophages. Journal of Bacteriology 136:668–681
    [Google Scholar]
  4. Davies R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Davis M. G., Calvo J. M. 1977; Isolation and characterization of λp leu bacteriophages. Journal of Bacteriology 129:1078–1090
    [Google Scholar]
  6. Gottesman M. E., Yarmolinsky M. B. 1968a; Integration-negative mutants of bacteriophage lambda. Journal of Molecular Biology 31:487–505
    [Google Scholar]
  7. Gottesman M. E., Yarmolinsky M. B. 1968b; The integration and excision of the bacteriophage lambda genome. Cold Spring Harbor Symposia on Quantitative Biology 33:735–747
    [Google Scholar]
  8. Henderson P. J. F., Giddens R. A., Jones-Mortimer M. C. 1977; Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochemical Journal 162:309–320
    [Google Scholar]
  9. Jones-Mortimer M. C., Kornberg H. L. 1976; Uptake of fructose by the sorbitol phosphotransferase of Escherichia coli K12. Journal of General Microbiology 96:383–391
    [Google Scholar]
  10. Kornberg H. L., Watts P. D., Brown K. 1980; Mechanisms of ‘inducer exclusion’ by glucose. FEBS Letters 117: Supplement, K28–K36
    [Google Scholar]
  11. Kundig W., Ghosh S., Roseman S. 1964; Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system. Proceedings of the National Academy of Sciences of the United States of America 521067–1074
    [Google Scholar]
  12. Lengeler J. 1975; Mutations affecting transport of the hexitols d-mannitol, d-glucitol and galactitol in Escherichia coli K12: isolation and mapping. Journal of Bacteriology 124:26–38
    [Google Scholar]
  13. Lindahl G., Sironi G., Bialy G., Calendar R. 1970; Bacteriophage lambda: abortive infection of bacteria lysogenic for phage P2. Proceedings of the National Academy of Sciences of the United States of America 66587–594
    [Google Scholar]
  14. Markowitz A., Rosenbaum N. 1965; A regulator gene that is dominant on an episome and recessive on a chromosome. Proceedings of the National Academy of Sciences of the United States of America 541084–1091
    [Google Scholar]
  15. Mata M., Delstanche M., Robert-Badouy J. 1978; Isolation of specialised transducing bacteriophages carrying the structural genes of the hexuron ate system in Escherichia coli K12: exu region. Journal of Bacteriology 133:549–557
    [Google Scholar]
  16. Mcentee K. 1977; Genetic analysis of the Escherichia coli K12 srl region. Journal of Bacteriology 132:904–911
    [Google Scholar]
  17. Mcginnis J. F., Paigen K. 1969; Catabolite inhibition: a general phenomenon in the control of carbohydrate utilisation. Journal of Bacteriology 100:902–913
    [Google Scholar]
  18. Morse M. L., Lederberg E. M., Lederberg J. 1956; Transduction in Escherichia coli K12. Genetics 41:142–156
    [Google Scholar]
  19. Mousset S., Thomas R. 1968; Dilysogenic excision: an accessory expression of the termination function?. Cold Spring Harbor Symposia on Quantitative Biology 33:749–754
    [Google Scholar]
  20. Press R., Glansdorff N., Miner P., De Vries J., Kadner R., Maas W. K. 1971; Isolation of transducing particles of ɸ80 bacteriophage that carry different regions of the Escherichia coli genome. Proceedings of the National Academy of Sciences of the United States of America 68795–798
    [Google Scholar]
  21. Roberton A. M., Sullivan P. A., Jones-Mortimer M. C., Kornberg H. L. 1980; Two genes affecting glucarate utilization in Escherichia coli K12. Journal of General Microbiology 117:377–382
    [Google Scholar]
  22. Shimada K., Weisberg R. A., Gottesman M. E. 1972; Prophage A at unusual chromosomal locations. I. Location of the secondary attachment sites and properties of the lysogens. Journal of Molecular Biology 63:483–503
    [Google Scholar]
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