@article{mbs:/content/journal/micro/10.1099/00221287-128-3-605, author = "Boronat, A. and Jones-Mortimer, M. C. and Kornberg, H. L.", title = "A Specialized Transducing Phage, λpsrlA, for the Sorbitol Phosphotransferase of Escherichia coli K12", journal= "Microbiology", year = "1982", volume = "128", number = "3", pages = "605-611", doi = "https://doi.org/10.1099/00221287-128-3-605", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-3-605", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "A specialized transducing phage for the srlA gene, specifying the sorbitol-specific Enzyme II of the phosphoenolpyruvate: sugar phosphotransferase system, was constructed and its DNA was analysed by restriction endonuclease digestion. Phage construction involved four steps: (1) integration of λ into the srlA gene; (2) selection of phage carrying (a) the left and (b) the right end of the srlA gene by means independent of the function of the new DNA acquired; (3) reconstitution of the srlA gene in a dilysogen of these two phage; and (4) the excision, using the heteroimmune lambdoid phage 21, of a plaque-forming srlA + phage from the dilysogenic chromosome. Comparison of the DNA restriction digests of the transducing phage with those of its parents and of wild-type λ revealed fragments consisting partly of λ and partly of Escherichia coli DNA. The junction points in the intermediate phage define a site that must lie within the reconstituted gene of the final phage. This technique should be of general application in relating genes, cloned by our method, to DNA sequences.", }