1887

Abstract

Mitochondria from conidia of were isolated and purified by a simple and rapid technique. Electron micrographic analysis and isopycnic centrifugation on a sucrose density gradient revealed a heterogeneous population.

Succinate, citrate, 2-oxoglutarate, NADH and ascorbate + tetramethyl--phenylenediamine (TMPD) were oxidized by the mitochondria. Oxygen consumption was totally inhibited by antimycin A or cyanide. The electron transport chain was coupled to sites 1 and 2 of phosphorylation. Oligomycin, atractyloside and 2,4-dinitrophenol prevented energy coupling.

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/content/journal/micro/10.1099/00221287-127-2-309
1981-12-01
2021-05-07
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