%0 Journal Article %A Ortega Perez, Ruben %A Micha-Hamzehpour, Mehrbanou %A Turian, Gilbert %T Respiratory Properties of Mitochondria Isolated from Conidia of Neurospora crassa %D 1981 %J Microbiology, %V 127 %N 2 %P 309-314 %@ 1465-2080 %R https://doi.org/10.1099/00221287-127-2-309 %I Microbiology Society, %X Mitochondria from conidia of Neurospora crassa were isolated and purified by a simple and rapid technique. Electron micrographic analysis and isopycnic centrifugation on a sucrose density gradient revealed a heterogeneous population. Succinate, citrate, 2-oxoglutarate, NADH and ascorbate + tetramethyl-p-phenylenediamine (TMPD) were oxidized by the mitochondria. Oxygen consumption was totally inhibited by antimycin A or cyanide. The electron transport chain was coupled to sites 1 and 2 of phosphorylation. Oligomycin, atractyloside and 2,4-dinitrophenol prevented energy coupling. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-127-2-309