1887

Abstract

Two mutants of were isolated which differ from their respective parental strains in their growth responses to guanine and xanthine. Both mutants had purine phosphoribosyltransferase activities similar to their parental strains. One mutant, CB-3, had a lower guanine uptake rate apparently caused by a genetic lesion in a specific gene (designated ) responsible for facilitating the transport of guanine. This gene mapped at 3·5 min in the sequence The second mutant, GP103, had a purine carrier molecule with altered specificity, as demonstrated by a competition between hypoxanthine and xanthine for uptake.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-2-357
1980-12-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/2/mic-121-2-357.html?itemId=/content/journal/micro/10.1099/00221287-121-2-357&mimeType=html&fmt=ahah

References

  1. Alford B. L., Barnes E. M. 1976; Hypoxanthine transport by cultured Chinese hamster lung fibroblasts. Journal of Biological Chemistry 251:4823–4827
    [Google Scholar]
  2. Benson C. E., Gots J. S. 1975; Genetic modification of substrate specificity of hypoxan-thine phosphoribosyltransferase in Salmonella typhimurium. Journal of Bacteriology 121:77–82
    [Google Scholar]
  3. Benson C. E., Brehmeyer B. A., Gots J. S. 1971; Requirement of cyclic AMP for induction of GMP reductase in Escherichia coli. Biochemical and Biophysical Research Communications 43:1089–1094
    [Google Scholar]
  4. Berlin R. D. 1970; Specificities of transport systems and enzymes. Science 168:1539–1545
    [Google Scholar]
  5. Berlin R. D., Oliver J. M. 1975; Membrane transport of purine and pyrimidine bases and nucleosides in animal cells. International Review of Cytology 42:287–336
    [Google Scholar]
  6. Berlin R. D., Stadtman E. R. 1966; A possible role of purine nucleotide pyrophosphorylases in the regulation of purine uptake by Bacillus subtilis. Journal of Biological Chemistry 241:2679–2686
    [Google Scholar]
  7. Burton K. 1977; Transport of adenine, hypoxanthine and uracil into Escherichia coli. Biochemical Journal 168:195–204
    [Google Scholar]
  8. Chou J. Y., Martin R. G. 1972; Purine phosphoribosyltransferases of Salmonella typhimurium. Journal of Bacteriology 112:1010–1013
    [Google Scholar]
  9. Ely B., Weppelman R. M., Massey H. C. Jr Hartman P. E. 1974; Some improved methods in P22 transduction. Genetics 76:625–631
    [Google Scholar]
  10. Foster J. W., Moat A. G. 1978; Mapping and characterization of the nad genes in Salmonella typhimurium LT-2. Journal of Bacteriology 133:775–779
    [Google Scholar]
  11. Gots J. S., Benson C. E., Shumas S. R. 1972; Genetic separation of hypoxanthine and guaninexanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium. Journal of Bacteriology 112:910–916
    [Google Scholar]
  12. Hochstadt-Ozer J., Stadtman E. R. 1971a; The regulation of purine utilization in bacteria. H. Adenine phosphoribosyltransferase in isolated membrane preparations and its role in transport of adenine across the membrane. Journal of Biological Chemistry 246:5304–5311
    [Google Scholar]
  13. Hochstadt-Ozer J., Stadtman E. R. 1971b; The regulation of purine utilization in bacteria. III. The involvement of purine phosphoribosyl-transferases in the uptake of adenine and other nucleic acid precursors by intact resting cells. Journal of Biological Chemistry 246:5312–5320
    [Google Scholar]
  14. Jackman L. E., Hochstadt J. 1976; Regulation of purine utilization in bacteria. IV. Character-ization of hypoxanthine and guanine uptake into isolated membrane vesicles from Salmonella typhimurium. Journal of Bacteriology 126:312–326
    [Google Scholar]
  15. Langley D., Guest J. R. 1974; Biochemical and genetic characteristics of deletion and other mutant strains of Salmonella typhimurium LT2 lacking α-keto acid dehydrogenase complex activities. Journal of General Microbiology 82:319–335
    [Google Scholar]
  16. Langley D., Guest J. R. 1977; Biochemical genetics of the α-keto acid dehydrogenase complexes in Escherichia coli K12: isolation and biochemical properties of deletion mutants. Journal of General Microbiology 99:263–276
    [Google Scholar]
  17. Langley D., Guest J. R. 1978; Biochemical genetics of the α-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants. Journal of General Microbiology 106:103–117
    [Google Scholar]
  18. Low K. B. 1972; Escherichia coli K12 F-prime factors, old and new. Bacteriological Reviews 36:587–607
    [Google Scholar]
  19. Pickering W. R., Woods R. A. 1972; The uptake and incorporation of purines by wild-type Saccharomyces cerevisiae and a mutant resistant to 4-aminopyrazolo(3,4-d)pyrimidine. Biochimica et biophysica acta 264:45–58
    [Google Scholar]
  20. Rosner J. L. 1972; Formation, induction and curing of bacteriophage P1 lysogens. Virology 49:679–689
    [Google Scholar]
  21. Roy-Burman S., Visser D. W. 1975; Transport of purines and deoxyadenosine in Escherichia coli . Journal of Biological Chemistry 250:9270–9275
    [Google Scholar]
  22. Sanderson K. E., Hartman P. E. 1978; Linkage map of Salmonella typhimurium, edition V. Microbiological Reviews 42:471–519
    [Google Scholar]
  23. Sanderson K. E., Ross H., Ziegler L., Makela P. H. 1972; F+, Hfr and F′ strains of Salmonella typhimurium and Salmonella abony. Bacteriological Reviews 36:608–637
    [Google Scholar]
  24. Thakar J. H., Kalle G. P. 1968; Defective guanine uptake in an 8-azaguanine-resistant mutant of Salmonella typhimurium. Journal of Bacteriology 95:458–464
    [Google Scholar]
  25. Vogel H. J., Bonner D. M. 1956; Acetylorni- thinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  26. Zylka J. M., Plagemann P. G. W. 1975; Purine and pyrimidine transport by cultured Novikoff cells: specificities and mechanism of transport and relationship to phosphoribo-sylation. Journal of Biological Chemistry 250:5756–5767
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-2-357
Loading
/content/journal/micro/10.1099/00221287-121-2-357
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error