1887

Abstract

Changes in the cellular content of glucose 6-phosphate and storage carbohydrates during catabolite derepression were measured during the diauxic growth of on glucose (1 %, w/v) medium. After the exhaustion of glucose there were transient increases in glucose 6-phosphate, alkali-soluble glycogen and trehalose, reflecting enhanced gluconeogenesis, followed by an increase in acid-soluble glycogen. During the subsequent adaptation to growth on ethanol the amounts of glucose 6-phosphate, trehalose and glycogen returned to basal levels. Thus storage carbohydrates, of which glycogen is quantitatively the most important, appear to act as the energy source for catabolite derepression.

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/content/journal/micro/10.1099/00221287-113-1-195
1979-07-01
2021-07-23
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References

  1. Barwell C.J., Hess B. 1971; Regulation of pyruvate kinase during gluconeogenesis in Saccharomyces cerevisiae.. FEBS Letters 19:1–4
    [Google Scholar]
  2. Costa-Carvalho V.L.A., Panek A., Mattoon J.R. 1978; Effects of carbon source and genetic modifications of phosphoglucomutase in trehalose metabolism in Saccharomyces cerevisiae.. FEMS Microbiology Letters 4:221–224
    [Google Scholar]
  3. Haarasilta S., Oura E. 1975; On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of bakers’ yeast. The biphasic growth. European Journal of Biochemistry 52:1–7
    [Google Scholar]
  4. Küenzi M.T., Fiechter A. 1969; Changes in carbohydrate composition and trehalase activity during the budding cycle of Saccharomyces cerevisiae.. Archiv für Mikrobiologie 64:369–407
    [Google Scholar]
  5. Linnane A.W., Haslam J.M. 1970; The biogenesis of yeast mitochondria. Current Topics in Cellular Regulation 2:101–172
    [Google Scholar]
  6. Lowry O.H., Passonneu J.V. 1972 A Flexible System of Enzymatic Analysis. London & New York: Academic Press.;
    [Google Scholar]
  7. Mahler H.R., Lin C.C. 1978; Exogenous adenosine 3′: 5′-monophosphate can release yeast from catabolite repression. Biochemical and Biophysical Research Communications 83:1039–1047
    [Google Scholar]
  8. Maitra P.K., Lobo Z. 1978; Reversal of glycolysis in yeast. Archives of Biochemistry and Biophysics 185:535–543
    [Google Scholar]
  9. Manners D.J. 1971; The structure and biosynthesis of storage carbohydrates in yeast. In The Yeasts, 1st edn. 2 pp. 419–439 Rose A.H., Harrison J.S. Edited by London & New York: Academic Press.;
    [Google Scholar]
  10. Panek A. 1962; Synthesis of trehalose by bakers yeast (Saccharomyces cerevisiae). Archives of Biochemistry and Biophysics 98:349–355
    [Google Scholar]
  11. Panek A. 1963; Function of trehalose in bakers yeast (Saccharomyces cerevisiae). Archives of Biochemistry and Biophysics 100:422–425
    [Google Scholar]
  12. Panek A., Mattoon J.R. 1977; Regulation of energy metabolism in Saccharomyces cerevisiae. Relationship between catabolite repression, trehalose synthesis and mitochondrial development. Archives of Biochemistry and Biophysics 183:306–316
    [Google Scholar]
  13. Plaat J.B. Van Der, Solingen P. Van. 1974; Cyclic 3′5′-adenosine monophosphate stimulates trehalose degradation in bakers yeast. Biochemical and Biophysical Research Communications 56:580–587
    [Google Scholar]
  14. Polakis E.S., Bartley W. 1966; Changes in dry weight, protein, deoxyribonucleic acid, ribonucleic acid and reserve and structural carbohydrates during the aerobic growth cycle of yeast. Biochemical Journal 98:883–887
    [Google Scholar]
  15. Quain D.E., Haslam J.M. 1979; The effects of catabolite derepression on the accumulation of steryl esters and activity of β-hydroxymethyl-glutaryl-CoA reductase in Saccharomyces cerevisiae.. Journal of General Microbiology 111:343–351
    [Google Scholar]
  16. Stewart P.R. 1975; Analytical methods for yeasts. In Methods in Cell Biology 12 pp. 111–147 Prescott D.M. Edited by New York: Academic Press.;
    [Google Scholar]
  17. Trevelyan W.E., Harrison J.S. 1956; Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis and behaviour during anaerobic fermentation. Biochemical Journal 63:23–33
    [Google Scholar]
  18. Wijk R.Van, Konijn T.M. 1971; Cyclic 3′,5′- AMP in Saccharomyces carlsbergensis under various conditions of catabolite repression. FEBS Letters 13:184–186
    [Google Scholar]
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