1887

Abstract

Cervicovaginal diversity has been reported as a predictive biomarker for cervical cancer risk. We recently reported the bio-therapeutic potential of vaginal probiotics from healthy Indian women against vaginal pathogens, isolated from the invasive cervical cancer (ICC) patients.

The cervicovaginal microflora from cervical cancer patients has not yet been reported from Indian population.

The present study aimed at comparing the cervicovaginal microbiome between healthy controls (HC) and ICC patients from the Indian population.

In total, 30 vaginal swabs (15 from HC and 15 from ICC) were subjected to 16S rRNA gene sequencing. Alpha diversity was evaluated by Shannon and Chao1 index; and beta diversity by principle coordinate analysis (PCoA) of weighted and unweighted UniFrac distances. The relative abundance of the microbial taxa was done according to linear discriminant analysis effect size (LEfSe).

Predominance of spp. in ICC and in HC groups was observed. Alpha-diversity was found to be higher in ICC as compared to HC but was statistically non-significant. LEfSe analysis revealed and as the marker genera in ICC with a marked decrease in sp. Contrarily, in HC, and were found to be abundant.

Differences in the vaginal microbiome between healthy and ICC women could help in the early prediction of cervical cancer risk and thus in designing prevention strategies.

Funding
This study was supported by the:
  • Department of Science Technology, Govt. of India (Award SR/WOS-A/LS-205/2017 (G))
    • Principle Award Recipient: KambleAshwini
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/content/journal/jmm/10.1099/jmm.0.001819
2024-03-28
2024-04-22
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