1887

Abstract

SUMMARY

Broth-culture filtrates of induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70°C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 × 10. It was concluded that can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with remains to be determined.

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/content/journal/jmm/10.1099/00222615-26-2-93
1988-06-01
2022-05-28
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