@article{mbs:/content/journal/jmm/10.1099/00222615-26-2-93, author = "Leunk, R. D. and Johnson, P. T. and David, B. C. and Kraft, W. G. and Morgan, D. R.", title = "Cytotoxic activity in broth-culture filtrates of Campylobacter pylori", journal= "Journal of Medical Microbiology", year = "1988", volume = "26", number = "2", pages = "93-99", doi = "https://doi.org/10.1099/00222615-26-2-93", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-26-2-93", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "SUMMARY Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70°C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 × 103. It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.", }