- Volume 60, Issue 1, 1982
Volume 60, Issue 1, 1982
- Animal
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Production of Interferon-β by Human Lymphoblastoid Cells of T and Non-T-Non-B Lineages
More LessSUMMARYHuman lymphoblastoid cells of T and non-T-non-B lineages were examined for interferon (IFN) production induced by Sendai virus. All of the four T cell lines examined and two of the three non-T-non-B cell lines produced IFN-β, and the third non-T-non-B cell line, HL-60, a mixture of IFN-α and -β, in contrast to two B cell lines, which produced IFN-α.
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Production of High Titres of Interferon-γ by Cells Derived from Short-term Cultures of Murine Spleen Leukocytes in T-Cell Growth Factor-conditioned Medium
More LessSUMMARYShort-term culture of activated murine spleen cells in T-cell growth factor-conditioned medium resulted in a cell population that produced high titres of interferon-γ in response to the T-cell mitogen concanavalin A. Compared to interferon-γ production by freshly isolated spleen cells in response to the same mitogen, titres were 10 to 20 times higher at all cell densities tested. Furthermore, the minimal cell density at which interferon-γ production could be detected was significantly lower. The mitogen dose required for optimal interferon induction in this system was higher than in conventional spleen cell cultures.
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- Plant
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Physical mapping of the DNAs of Carnation Etched Ring and Figwort Mosaic Viruses
More LessSUMMARYThe native DNAs of both carnation etched ring virus (CERV) and figwort mosaic virus (FMV) formed multiple bands on gel electrophoresis thus resembling cauliflower mosaic virus (CaMV) DNA; there were some minor differences in banding patterns. Denaturation and nuclease S1 digestion showed that CERV DNA had three discontinuities (two in one strand and one in the other) and FMV DNA had four discontinuities (three in one strand and one in the other). These discontinuities and the sites of restriction endonuclease digestion were mapped on CERV and FMV DNAs and the maps compared with those of CaMV DNA. The maps of the three virus DNAs were very different.
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