1887

Abstract

SUMMARY

Some characteristics of a microplate method for the detection and assay of plant viruses using enzyme-labelled antibodies are described. The method enabled the highly sensitive detection of a number of morphologically different viruses in purified preparations and in unclarified extracts of herbaceous hosts and of infected crop plants. Virus concentrations were estimated by photometric measurement of the colour intensity of the hydrolysed substrate. The suitability of the technique for various field and research applications is considered.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-3-475
1977-03-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/3/JV0340030475.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-3-475&mimeType=html&fmt=ahah

References

  1. Avrameas S. 1969; Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry 6:43–52
    [Google Scholar]
  2. Bercks R. 1967; Methodische Untersuchungen über den Nachweis pflanzenpathologener Viren mit dem Bentonit-Flockungstest, dem Latex-Test und dem Bariumsulfat-Test. Phytopathologische Zeitschrift 58:1–17
    [Google Scholar]
  3. Engvall E., Perlmann P. 1971; Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
    [Google Scholar]
  4. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  5. Feldmann G., Druet P., Bignon J., Avrameas S. 1976; Immunoenzymatic techniques. In Proceedings of the First International Symposium on Immunoenzymatic Techniques p 512 Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  6. Jermoljev E., Albrechtova L. 1965; Aplikace suspenze latex v sérologické diagnóze virových chorob rostlin. Ochrana Rostlin 2:51–56
    [Google Scholar]
  7. Nakane P. K., Pierce G. B. 1966; Enzyme-labelled antibodies: preparation and application for the localization of antigens. The Journal of Histochemistry and Cytochemistry 14:929–931
    [Google Scholar]
  8. van Regenmortel M. V. H. 1964; Purification of plant viruses by zone electrophoresis. Virology 23:495–502
    [Google Scholar]
  9. van Weemen B. K., Schuurs A. H. W. M. 1971; Immunoassay using antigen-enzyme conjugates. FEBS Letters 15:232–235
    [Google Scholar]
  10. van Weemen B. K., Schuurs A. H. W. M. 1972; Immunoassay using hapten-enzyme conjugates. FEBS Letters 24:77–81
    [Google Scholar]
  11. Voller A., Bartlett A., Bid Well D. E., Clark M. F., Adams A. N. 1976a; The detection of viruses by enzyme-linked immunosorbent assay (ELISA). Journal of General Virology 33:165–167
    [Google Scholar]
  12. Voller A., Bid Well D. E., Bartlett A. 1976b; Enzyme immunoassays in diagnostic medicine: theory and practice. Bulletin of the World Health Organization 53:55–65
    [Google Scholar]
  13. Voller A., Bidwell D., Huldt G., Engvall E. 1974; A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bulletin of the World Health Organization 51:209–211
    [Google Scholar]
  14. Wicker R., Avrameas S. 1969; Localization of virus antigens by enzyme-labelled antibodies. Journal of General Virology 4:465–471
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-3-475
Loading
/content/journal/jgv/10.1099/0022-1317-34-3-475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error