1887

Abstract

SUMMARY

The effect of gangliosides extracted from human group O Rh+ erythrocytes on haemagglutination by BK virus was investigated. Experiments were performed on both ganglioside mixtures and isolated fractions separated by column chromatography and characterized by thin-layer chromatography. These results were compared with those obtained with standard preparations of gangliosides, and the inhibiting activity was shown to be confined mainly to gangliosides with a lower than GM1. It was also observed that the insertion of gangliosides in liposomes increased the haemagglutination-inhibiting activity and that ganglioside coating restored the ability of glycosidase- treated human red blood cells to agglutinate.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-3-879
1987-03-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/3/JV0680030879.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-3-879&mimeType=html&fmt=ahah

References

  1. Aminoff D. 1959; The determination of free sialic acid in the presence of the bound compound. Virology 7:355–357
    [Google Scholar]
  2. Ando S., Cheng N. C., Yu R. K. 1978; High-performance thin-layer chromatography and densitometrie determination of brain gangliosides composition of several species. Analytical Biochemistry 89:437–450
    [Google Scholar]
  3. Blough H. A. 1971; Attachment of myxoviruses to artificial lipoprotein membranes containing gangliosides. FEBS Abstract 7:204
    [Google Scholar]
  4. De Stasio A., Mastromarino P., Panà A., Seganti L., Sinibaldi L., Valenti P., Orsi N. 1980; Characterization of a glycoprotein inhibitor present in human serum and active towards BK virus hemagglutination. Microbiologica 3:293–305
    [Google Scholar]
  5. Dubois M., Giles K. A., Hamilton J. K., Rebers P. A. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (BK) isolated from urine after renal transplantation. Lancet i:1253–1257
    [Google Scholar]
  7. Haywood A. M. 1974a; Characteristics of Sendai virus receptors in a model membrane. Journal of Molecular Biology 83:427–436
    [Google Scholar]
  8. Haywood A. M. 1974b; Fusion of Sendai virus with model membranes. Journal of Molecular Biology 87:625–628
    [Google Scholar]
  9. Holmgren J., Svennerholm L., Elwing H., Fredman P., Stannegard O. 1980; Sendai virus receptor: proposed recognition structure based on binding to plastic adsorbed gangliosides. Proceedings of the National Academy of Sciences U.S.A.: 771947–1950
    [Google Scholar]
  10. Markwell M. A., Svennerholm L., Paulson J. C. 1981; Specific gangliosides function as host cell receptors for Sendai virus. Proceedings of the National Academy of Sciences U.S.A.: 785406–5410
    [Google Scholar]
  11. Markwell M. A., Fredman P., Svennerholm L. 1984; Receptor ganglioside content of three hosts for Sendai virus. Biochimica et biophysica acta 775:7–16
    [Google Scholar]
  12. Roche A. C., Maget-Dane R., Obrenovich A., Hildebrand K., Nicolau C., Monsigny M. 1978; Interaction between vesicles containing gangliosides and limulin (Limulus poliphemus lectin). Fluorescence polarization of l,6-diphenyl-l,3,5-hextriene. FEBS Letters 93:91–96
    [Google Scholar]
  13. Seganti L., Mastromarino P., Panà A., De Stasio A., Sinibaldi L. 1980; Inhibitory activity of blood serum components towards BK virus. Abstract of the XIII FEBS Meeting p 226 Jerusalem: 24-29 August 1980
    [Google Scholar]
  14. Seganti L., Mastromarino P., Superti F., Panà A., Orsi N. 1981a; Comparative study of non-antibody inhibitors present in plasma of different animals and active towards BK virus, a human papovavirus. Microbiologica 4:395–402
    [Google Scholar]
  15. Seganti L., Mastromarino P., Superti F., Sinibaldi L., Orsi N. 1981b; Receptors for BK on human erythrocytes. Acta virologica 25:177–181
    [Google Scholar]
  16. Sharom F. J., Barratt D. G., Thede A. E., Grand C. W. N. 1976; Glycolipids in model membranes spin label and freeze-etch studies. Biochimica et biophysica acta 455:485–492
    [Google Scholar]
  17. Sinibaldi L., Goldoni P., Seganti L., Superti F., Tsiang H., Orsi N. 1985; Gangliosides in early interactions between vesicular stomatitis virus and CER cells. Microbiologica 8:355–365
    [Google Scholar]
  18. Superti F., Hauttecoeur B., Morelec M. J., Goldoni P., Bizzini B. 1986; Involvement of gangliosides in rabies virus infection. Journal of General Virology 67:47–56
    [Google Scholar]
  19. Suzuki Y., Suzuki T., Matsunaga M., Matsumoto M. 1985; Gangliosides as paramyxovirus receptors. Structural requirement of sialo-oligosaccharides in receptors for hemagglutinating virus of Japan (Sendai virus) and Newcastle disease virus. Journal of Biochemistry 97:1189–1199
    [Google Scholar]
  20. Svennerholm L. 1957; Quantitative estimation of sialic acids. II. A. colorimetric resorcinol-hydrochloric acid method. Biochimica et biophysica acta 24:604–611
    [Google Scholar]
  21. Tettamanti G., Bonali F., Marchesini S., Zambotti V. 1973; A new procedure for the extraction, purification and fractionation of brain gangliosides. Biochimica et biophysica acta 296:160–170
    [Google Scholar]
  22. Umeda M., Nojima S., Inoue K. 1984; Activity of human erythrocyte gangliosides as a receptor for HVJ. Virology 133:172–182
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-3-879
Loading
/content/journal/jgv/10.1099/0022-1317-68-3-879
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error