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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 4

gen. nov., sp. nov., a novel taxon within the family isolated from sediment of Qiantang River No Access

Abstract

A Gram-stain-negative bacterium, designated LG-4, was isolated from sediment of Qiantang River in Zhejiang Province, PR China. Cells were strictly aerobic, non-spore-forming, non-motile and short-rod-shaped (1.0–1.2 µm long and 0.7–0.8 µm wide). Growth occurred at 15–42 °C (optimum, 30 °C), at pH 5.0–9.0 (pH 7.0) and at 0–2.0 % (w/v) NaCl (optimum, 0.5 % NaCl). Strain LG-4 showed 95.75–96.90 % 16S rRNA gene sequence similarity to various type strains of the genera , , , and of the family , and the most closely related strain was ZQBW (96.90 % similarity). The phylogenomic tree showed that strain LG-4 clustered in the family and was positioned outside of the clade composed of the genera and . The average nucleotide identity and digital DNA–DNA hybridization values between strain LG-4 and the related type strains were in the range of 74.19–77.56 % and 16.70–25.80 %, respectively. The average amino acid identity (AAI) values between strain LG-4 and related type strains of the family were 60.94–69.73 %, which are below the genus boundary (70 %). The evolutionary distance (ED) values between LG-4 and the related genera of the family were 0.21–0.34, which are within the recommended standard (≥0.21–0.23) for defining a novel genus in the family . The predominant cellular fatty acids were C ω7, C cyclo ω8, C and C, the isoprenoid quinone was Q-10, and the major polar lipids were phospholipid, phosphatidylglycerol, phosphatidylcholine, aminolipid and two unknown polar lipids. The genome size was 4.7 Mb with 68.6 mol% G+C content. On the basis of distinct phylogenetic relationships, low AAI values and high ED values, and differential phenotypic, physiological and biochemical characteristics, strain LG-4 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LG-4 (=MCCC 1K08849=KCTC 8136)

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , AAI , ANI , dDDH , polyphasic taxonomy and Ruixingdingia sedimenti gen. nov., sp. nov.
Funding
This study was supported by the:
  • Science and Technology Program of Gansu Province (Award 22YF7FG188, 22JR5RG565)
    • Principle Award Recipient: luxu
  • Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science (Award BM2022019)
    • Principle Award Recipient: luxu
  • National Natural Science Foundation of China (Award 32170128)
    • Principle Award Recipient: NotApplicable
© 2024 The Authors
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2024-04-26
2025-01-23
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Ruixingdingia sedimenti gen. nov., sp. nov., a novel taxon within the family Paracoccaceae isolated from sediment of Qiantang River
Int J Syst Evol Microbiol 74, 006350 (2024); https://doi.org/10.1099/ijsem.0.006350
/content/journal/ijsem/10.1099/ijsem.0.006350
/content/journal/ijsem/10.1099/ijsem.0.006350
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