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Volume 74, Issue 4

gen. nov., sp. nov., a member of a novel genus of the family , isolated from the gut of the western honey bee Open Access

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, CA-0114, was isolated from the midgut of a western honey bee, . The isolate exhibited ≤96.43 % 16S rRNA gene sequence identity (1540 bp) to members of the families and . Phylogenetic trees based on genome distance phylogeny and concatenated protein sequences encoded by conserved genes , , , , and separated the isolate from other genera forming a distinct lineage in the . In both trees, the closest relatives were YMB-R21 and BIT-L3, which were isolated previously from L., a plastic-eating mealworm. Digital DNA–DNA hybridization, orthologous average nucleotide identity and average amino acid identity values between strain CA-0114 and the closest related members within the were ≤23.1, 75.45 and 76.04 %, respectively. The complete genome of strain CA-0114 was 4 451669 bp with a G+C content of 52.12 mol%. Notably, the apparent inability of strain CA-0114 to ferment -glucose, inositol and -rhamnose in the API 20E system is unique among closely related members of the . Based on the results obtained through genotypic and phenotypic analysis, we propose that strain CA-0114 represents a novel species and genus within the family , for which we propose the name gen. nov., sp. nov. (type strain CA-0114=ATCC TSD-396=DSM 116385).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Apirhabdus apintestini , Apis mellifera , CA-0114T and Enterobacteriaceae
Funding
This study was supported by the:
  • Canada First Research Excellence Fund (Award THE3-00525)
    • Principle Award Recipient: EmmaAllen-Vercoe
  • CIHR (Award PDF-402947-2023)
    • Principle Award Recipient: BrendanA Daisley
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-03
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Apirhabdus apintestini gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the western honey bee Apis mellifera
Int J Syst Evol Microbiol 74, 006346 (2024); https://doi.org/10.1099/ijsem.0.006346
/content/journal/ijsem/10.1099/ijsem.0.006346
/content/journal/ijsem/10.1099/ijsem.0.006346
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