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Volume 74, Issue 4

sp. nov., a novel indole-producing and cellulose-degrading bacterium isolated from soil No Access

Abstract

A Gram-stain-negative, rod-shaped, indole-producing, and cellulose-degrading bacterial strain, designated NEAU-G-C5, was isolated from soil collected from a forest in Dali city, Yunnan province, south China. 16S rRNA gene sequence analysis showed that strain NEAU-G-C5 was assigned to the genus and showed high sequence similarities to 12-OD1 (98.32 %) and 6 NM-7 (98.41 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G-C5 formed a lineage related to 12-OD1 and 6 NM-7. The major fatty acids of the strain were C, C ω7, and C cyclo. The respiratory quinone was Q-8. The polar lipid profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. In addition, the average nucleotide identity values between strain NEAU-G-C5 and its reference strains 12-OD1, 6 NM-7, NS9, and TSA1 were 89.7, 88.2, 81.3, and 88.0 %, respectively, and the levels of digital DNA–DNA hybridization between them were found to be 58.5 % (54.9–62.0 %), 53.2 % (49.8–56.7 %), 31.9 % (28.6–35.5 %), and 57.7 % (54.1–61.2 %), respectively, which were lower than the accepted threshold values of 95–96 % and 70 %, respectively. The DNA G+C content of strain NEAU-G-C5 was 66.5 mol%. The strain could produce indoleacetic acid and cellulase. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, we conclude that strain NEAU-G-C5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-G-C5 (=MCCC 1K08668=KCTC 8080).

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  • Accepted:
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Keyword(s): indole-producing , Massilia luteola sp. nov. , 16S rRNA gene , cellulose-degrading and genome characterization
Funding
This study was supported by the:
  • Natural Science Foundation of Heilongjiang Province (Award TD2022C002)
    • Principle Award Recipient: WenshengXiang
  • Key Programme (Award 32030090)
    • Principle Award Recipient: WenshengXiang
  • Key Programme (Award U22A20483)
    • Principle Award Recipient: XiangjingWang
© 2024 The Authors
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2024-04-29
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Massilia luteola sp. nov., a novel indole-producing and cellulose-degrading bacterium isolated from soil
Int J Syst Evol Microbiol 74, 006331 (2024); https://doi.org/10.1099/ijsem.0.006331
/content/journal/ijsem/10.1099/ijsem.0.006331
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