Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand

Pannida Khunnamwong; Sasitorn Jindamorakot; Somjit Am-In; Varunya Sakpuntoon; Nantana Srisuk; Pumin Nutaratat; Wanatchaporn Boontham; Savitree Limtong

doi:10.1099/ijsem.0.006330

Volume 74, Issue 4

Research Article

Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand

Pannida Khunnamwong^1,2, Sasitorn Jindamorakot³, Somjit Am-In³, Varunya Sakpuntoon¹, Nantana Srisuk^1,2, Pumin Nutaratat^4,5, Wanatchaporn Boontham⁶ and Savitree Limtong^1,2,7
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Affiliations: ¹ Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand ² Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand ³ Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Luang, Pathum Thani 12120, Thailand ⁴ Department of Biology, Faculty of Science and Digital Innovation, Thaksin University, Pa Phayom, Phatthalung 93210, Thailand ⁵ Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science and Digital Innovation, Thaksin University, Pa Phayom, Phatthalung 93210, Thailand ⁶ Program of Microbiology, Faculty of Science and Technology, Nakhon Pathom Rajabhat University, Nakhon Pathom 73000, Thailand ⁷ Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand
*Correspondence: Savitree Limtong, [email protected]
Published: 09 April 2024 https://doi.org/10.1099/ijsem.0.006330

Abstract

Four yeast strains, representing a novel anamorphic species, were isolated in Thailand. The two strains (ST-3660T and ST-3647) were obtained from two different estuarine water samples in a mangrove forest. Strain DMKU-FW1-37 was derived from a grease sample, and another strain (TSU57) was isolated from a fruiting body of Phallus sp. Pairwise sequence analysis showed that the four strains had identical or differed by only one nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene, and differed by one to three nucleotide substitutions in the internal transcribed spacer (ITS) regions. Savitreea pentosicarens is the most closely related species to the four strains, but with 9–10 (1.57–1.72 %) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 29–31 (4.22–4.45 %) nucleotide substitutions in the ITS regions. Phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene showed that the four strains form a well-separated lineage from S. pentosicarens with high bootstrap support, confirming that they represent a distinct species. Therefore, the four strains are assigned as representives of a novel species of the genus Savitreea, for which the name Savitreea siamensis sp. nov. is proposed. The holotype is TBRC 4481T and the ex-type is PYCC 9794T (=ST-3660T). The MycoBank number of the novel species is MB 851951.

Received: 13/02/2024
Accepted: 28/03/2024
Published Online: 09/04/2024

Keyword(s): food waste trap , mangrove forest , mushroom , Savitreea siamensis , Thailand and yeast

Funding

This study was supported by the:

Institute for Advanced Studies, Kasetsart University (Award Grant No. FF(KU)18.64)
- Principle Award Recipient: NantanaSrisuk
Kasetsart University for research funding through the Biodiversity Center Kasetsart University
- Principle Award Recipient: PannidaKhunnamwong

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Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand

Int J Syst Evol Microbiol 74, 006330 (2024); https://doi.org/10.1099/ijsem.0.006330

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Volume 74, Issue 4

Research Article

Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand

Abstract

Funding

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