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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

sp. nov., a moderately halotolerant bacterium isolated from intertidal sediment No Access

Abstract

A Gram-stain-negative, aerobic, rod-shaped and halotolerant bacterium, designated as strain ASW11-75, was isolated from intertidal sediments in Qingdao, PR China, and identified using a polyphasic taxonomic approach. Growth of strain ASW11-75 occurred at 10–45 °C (optimum, 37 °C), pH 6.5–9.0 (optimum, pH 8.0) and 0.5–18.0 % NaCl concentrations (optimum, 2.5 %). Phylogenetic analyses based on 16S rRNA gene sequences and 1179 single-copy orthologous clusters indicated that strain ASW11-75 is affiliated with the genus . Strain ASW11-75 showed highest 16S rRNA gene sequence similarity to ‘’ CAU 1620 (98.5 %). The digital DNA–DNA hybridization and average nucleotide identity values between strain ASW11-75 and its closely related strains ( R9SW1, A3d10, ‘’ CAU 1620, R65, Hb8, LZ-8 and ATCC 49840) were 19.8–24.5 % and 76.6–80.7 %, respectively. The predominant cellular fatty acids were C, C 9 and C N alcohol. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid and two unidentified lipids. The major isoprenoid quinone was ubiquinone-9. The genomic DNA G+C content was 62.2 mol%. Based on genomic and gene function analysis, strain ASW11-75 had lower protein isoelectric points with higher ratios of acidic residues to basic residues and possessed genes related to ion transport and organic osmoprotectant uptake, implying its potential tolerance to salt. The results of polyphasic characterization indicated strain ASW11-75 represents a novel species, for which the name sp. nov. with the type strain ASW11-75 is proposed. The type strain is ASW11-75 (=KCTC 82497=MCCC 1K05587).

  • Received:
  • Accepted:
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Keyword(s): halotolerant , intertidal sediment , Marinobacter and polyphasic taxonomy
Funding
This study was supported by the:
  • Shanxi Agricultural University (Award 2020BQ39)
    • Principle Award Recipient: YiLi
  • Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi Province (Award 2021L134)
    • Principle Award Recipient: YiLi
  • International Science and Technology Cooperation Program of Shanxi Province (Award 20210302124004)
    • Principle Award Recipient: YiLi
  • National Natural Science Foundation of China (Award 32300115)
    • Principle Award Recipient: YiLi
  • National Natural Science Foundation of China (Award 32300037)
    • Principle Award Recipient: XuanShao
  • Doctoral Research Foundation of Shangqiu Normal University (Award 7001-700168)
    • Principle Award Recipient: XuanShao
© 2024 The Authors
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Marinobacter qingdaonensis sp. nov., a moderately halotolerant bacterium isolated from intertidal sediment
Int J Syst Evol Microbiol 74, 006327 (2024); https://doi.org/10.1099/ijsem.0.006327
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