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Volume 74, Issue 4

sp. nov., a novel yellow bacterium isolated from soil No Access

Abstract

An aerobic, non-motile, Gram-stain-positive bacterium, designated strain NEAU-Y5, was isolated from a soil sample collected from Northeast Agricultural University, Heilongjiang province. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Y5 belonged to the genus and showed high 16S rRNA sequence similarity to (98.9 %), (98.9 %), (98.5 %), (98.5 %), (98.5 %), (98.3 %), and (98.1 %), followed by other members of the genus (<98 %), and phylogenetically clustered with and . Strain NEAU-Y5 was found to grow at 4–40 °C (optimum, 28 °C), pH 6.0–12.0 (optimum, pH 7.0), and tolerated 0–6 % NaCl (w/v). The cell-wall peptidoglycan type was -Lys–-Asp. The whole-cell hydrolysates contained glucose, galactose, and ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, and glucosamine unknown phospholipid. Major fatty acids were anteiso-C and anteiso-C. The predominant menaquinone was MK-9(H). The DNA G+C content was 73.4 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain NEAU-Y5 and the type strains of the genus ranged from 18.6 to 23.5 % and from 77.3 to 81.6 %, respectively. Based on morphological, physiological, chemotaxonomic, and phylogenetic data, as well as digital DNA–DNA hybridization and average nucleotide identity values, the novel strain NEAU-Y5 could be differentiated from its closest relatives. Therefore, the strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-Y5 (=CCTCC AA 2019087=DSM 110637).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , Isoptericola luteus sp. nov. , polyphasic taxonomy , soil and whole genome
Funding
This study was supported by the:
  • Heilongjiang Provincial Postdoctoral Science Foundation (Award LBH-Q21072)
    • Principle Award Recipient: JunweiZhao
  • Natural Science Foundation of Heilongjiang Province (Award TD2022C002)
    • Principle Award Recipient: WenshengXiang
  • Outstanding Youth Project of Natural Science Foundation of Heilongjiang Province (Award YQ2021C012)
    • Principle Award Recipient: JunweiZhao
  • Key Programme (Award 32030090)
    • Principle Award Recipient: WenshengXiang
© 2024 The Authors
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2024-04-29
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Isoptericola luteus sp. nov., a novel yellow bacterium isolated from soil
Int J Syst Evol Microbiol 74, 006324 (2024); https://doi.org/10.1099/ijsem.0.006324
/content/journal/ijsem/10.1099/ijsem.0.006324
/content/journal/ijsem/10.1099/ijsem.0.006324
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