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Volume 74, Issue 4

sp. nov., a member of the isolated from sandstone collected from the Sahara Desert in Tunisia No Access

Abstract

The taxonomic position of strain BMG 8361, isolated from sandstone collected in the Sahara Desert of Southern Tunisia, was refined through a polyphasic taxonomic investigation. Colonies of BMG 8361 were pale-orange coloured, irregular with a dry surface and produced a diffusible pink or brown pigment depending on media. The Gram-positive cells were catalase-positive and oxidase-negative. The strain exhibited growth at 10–40 °C and pH values ranging from 5.5 to 9.0, with optima at 28–35 °C and pH 6.5–8.0. Additionally, BMG 8361 demonstrated the ability to grow in the presence of up to 1 % NaCl (w/v) concentration. The peptidoglycan of the cell wall contained -diaminopimelic acid, glucose, galactose, xylose, ribose, and rhamnose. The predominant menaquinones consisted of MK-9(H) and MK-9. The main polar lipids were phosphatidylcholine, phosphatidylinositol, glycophosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified lipids. Major cellular fatty acids were iso-C, iso-C h, and C 8. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain BMG 8361 within the genus . The highest pairwise sequence similarity observed in the 16S rRNA gene was 99.5 % with AT 7-14. However, when considering digital DNA–DNA hybridization and average nucleotide identity, the highest values, 48.4 and 86.58 %, respectively, were obtained with BMG 822. These values significantly undershoot the recommended thresholds for establishing new species, corroborating the robust support for the distinctive taxonomic status of strain BMG 8361 within the genus . In conjunction with the phenotyping results, this compelling evidence leads to the proposal of a novel species we named sp. nov. with BMG 8361 (=DSM 46845=CECT 8880) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): diffusible brown pigment , Geodermatophilaceae , polyphasic taxonomy , stone-dwelling actinobacteria and taxogenomics
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2024-04-29
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Blastococcus brunescens sp. nov., a member of the Geodermatophilaceae isolated from sandstone collected from the Sahara Desert in Tunisia
Int J Syst Evol Microbiol 74, 006317 (2024); https://doi.org/10.1099/ijsem.0.006317
/content/journal/ijsem/10.1099/ijsem.0.006317
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