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Volume 74, Issue 4

sp. nov. and sp. nov., isolated from surface seawater of the Pacific Ocean No Access

Abstract

Two Gram-negative, non-spore-forming, non-motile, non-flagellated bacteria, designated strains D6 and DH64, were isolated from surface water of the Pacific Ocean. For strain D6, growth occurred at 10–40 °C, pH 5.5–9.0 and in the presence of 0–8.0 % NaCl (w/v). For strain DH64, growth occurred at 10–40 °C, pH 5.5–8.5 and in the presence of 0.5–8.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains D6 and DH64 both belonged to the genera , with the highest sequence identities to JCM 17757 (98.2 %) and JCM 11811 (98.6 %), respectively. The 16S rRNA gene sequence identity between strains D6 and DH64 was 95.9 %. The average amino acid identity and digital DNA–DNA hybridization values between the two strains and the nearest phylogenetic neighbours were 66.7–93.3 % and 16.1–38.5 %, respectively. The major respiratory quinone of both strains was menaquinone-6. The major polar lipid was phosphatidylethanolamine. The major fatty acids were identified similarly as iso-C G, iso-C and iso-C 3-OH. The genomic G+C contents of strains D6 and DH64 were determined to be 45.5 and 42.6 mol%, respectively. The combined genotypic and phenotypic data show that the strains represent two novel species within genera , for which the names sp. nov. and sp. nov. are proposed, with type strains D6 (=MCCC M28982=KCTC 92604) and DH64 (=MCCC M28986=KCTC 92975).

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  • Accepted:
  • Published Online:
Keyword(s): East Pacific Ocean , Flagellimonas baculiformis , Flagellimonas crocea and surface seawater
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2022YFC2804005)
    • Principle Award Recipient: Ge-YiFu
© 2024 The Authors
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Flagellimonas baculiformis sp. nov. and Flagellimonas crocea sp. nov., isolated from surface seawater of the Pacific Ocean
Int J Syst Evol Microbiol 74, 006316 (2024); https://doi.org/10.1099/ijsem.0.006316
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