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Abstract

Strain LB-N7, a novel Gram-negative, orange, translucent, gliding, rod-shaped bacterium, was isolated from water samples collected from an open system of Atlantic salmon () smolts in a fish farm in Chile during a flavobacterial infection outbreak in 2015. Phylogenetic analysis based on 16S rRNA sequences (1337 bp) revealed that strain LB-N7 belongs to the genus and is closely related to the type strains A2-1 (98.8 %) and R2A45-3 (96.75 %). The genome size of strain LB-N7 was 2.93 Mb with a DNA G+C content 32.6 mol%. Genome comparisons grouped strain LB-N7 with NJ-26, HXWNR29, TH16-21 and TWA-26. The calculated digital DNA–DNA hybridization values between strain LB-N7 and the closest related strains were 23.3 % and the average nucleotide identity values ranged from 71.52 to 79.39 %. Menaquinone MK-6 was the predominant respiratory quinone, followed by MK-7. The major fatty acids were iso-C and anteiso-C. The primary polar lipids detected included nine unidentified lipids, two amounts of aminopospholipid and phospholipids, and a smaller amount of aminolipid. Phenotypic, genomic, and chemotaxonomic data suggest that strain LB-N7 (=CECT 30406=RGM 3221) represents as a novel bacterial species, for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Agencia Nacional de Investigación y Desarollo (ANID, Chile) (Award INCAR FONDAP 1522A0004 and 1523A0007)
    • Principle Award Recipient: Avendaño-HerreraRuben
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-04-02
2024-04-23
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